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首页> 外文期刊>Current Problems in Cancer >Microsatellite instability profiling in Egyptian bladder cancer patients: A pilot study
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Microsatellite instability profiling in Egyptian bladder cancer patients: A pilot study

机译：埃及膀胱癌患者微卫星不稳定性分析：试验研究

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Microsatellite alterations have been implicated in the pathogenesis of many cancers; however, they are still not well addressed in the bladder cancer (BC) of Egyptian population. We assessed microsatellite instability (MSI) profile and loss of heterozygosity (LOH) using 13 microsatellite markers in tumor tissue samples and urine sediments obtained from 30 Egyptian patients with BC. The concordance between MSI in tumor tissue and urine samples was determined, and correlated to relevant clinicopathologic features. We found that MSI was more frequent than LOH (100% and 46.7%, respectively). D16S310, MBP, and IFN-alpha showed the highest MSI frequency in urine samples (70%, 70%, and 66.67%, respectively), while MBP, ACTBP2, and D9S171 (66.67%, 63.33%, and 60%, respectively) were the most frequently detected in tumor tissues. All assessed MSI markers correlated significantly with pathologic subtype (being more frequent in TCC) and with hematuria. The concordance between tissue and urine samples was statistically significant for D16S476, D9S171, FGA, and ACTBP2 (P=0.04, 0.015, 0.02, and 0.007, respectively). When we combined D16S476 and D9S171, the sensitivity, specificity, positive predictive value, and negative predictive value for the diagnosis of BC were 80.0%, 75.0%, 82.8%, and 71.4%, respectively. Accordingly, we concluded that MSI in urine sediments could be a potential tool for the diagnosis of BC. (C) 2019 Elsevier Inc. All rights reserved.
机译：微卫星改变涉及许多癌症的发病机制;然而，它们仍然在埃及人口的膀胱癌（BC）中仍然不好。我们评估了使用肿瘤组织样品中的13个微卫星标志物和从30名埃及BC患者获得的尿疏水素标志物评估微卫星不稳定性（MSI）概况和杂合性（LOH）的丧失。测定肿瘤组织和尿液样品中MSI之间的一致性，并与相关的临床病理特征相关。我们发现MSI比LOH更频繁（分别为100％和46.7％）。 D16S310，MBP和IFN-alpha在尿液样本中显示出最高的MSI频率（分别为70％，70％和66.67％），而MBP，ACTBP2和D9S171（分别为66.67％，63.33％和60％）在肿瘤组织中最常检测到。所有评估的MSI标记显着与病理亚型（在TCC中更频繁）和血尿有关。组织和尿液样品之间的一致性对于D16S476，D9S171，FGA和ACTBP2分别具有统计学意义（P = 0.04,0.02和0.007）。当我们组合D16S476和D9S171时，敏感性，特异性，阳性预测值和BC诊断的负预测值分别为80.0％，75.0％，82.8％和71.4％。因此，我们得出结论，尿沉积物中的MSI可能是诊断BC的潜在工具。 （c）2019 Elsevier Inc.保留所有权利。

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