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首页> 外文期刊>Current Protocols in Cell Biology >Using Acutely Dissociated and PurifiedOligodendrocyte Precursor Cells forHigh-Throughput Drug Screening toIdentify Compounds that PromoteOligodendrocyte Differentiation
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Using Acutely Dissociated and PurifiedOligodendrocyte Precursor Cells forHigh-Throughput Drug Screening toIdentify Compounds that PromoteOligodendrocyte Differentiation

机译：使用急性解离和纯化的摩洛哥肾上腺细胞前体细胞进行高通量的药物筛选造成的化合物，所述化合物称之见的化合物分化

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摘要

Multiple sclerosis (MS) is an autoimmune disease that involves an immunemediatedinflammatory response in the central nervous system and optic nerveresulting in demyelination and neural degeneration, the cause of which is unknown.The adult central nervous system has the capacity to remyelinate axonsby generating new oligodendrocytes (OLs). To identify clinical candidate compoundsthat may promote remyelination, we have developed a high-throughputscreening (HTS) assay to identify compounds that promote the differentiationof oligodendrocyte precursor cells (OPCs) into OLs. Using acutely dissociatedand purified rat OPCs coupled with immunofluorescent image quantification,we have developed an OL differentiation assay. Building on OPC culturing techniquesdeveloped over the past 30 years, we have scaled up the isolation andpurification process to generate sufficient quantities for HTS. We then describethe use of these acutely derived OPCs in an assay designed to identify compoundsthat promote differentiation into OLs. We have validated this assay witha known promoter of differentiation, thyroid hormone, and subsequently usedthe assay to screen the NIH clinical collection library (Lariosa-Willingham,et al., 2016).
机译：多发性硬化症（MS）是一种自身免疫性疾病，涉及中枢神经系统中的免疫硫化炎症反应和在脱髓鞘和神经变性中的视神经，其原因是未知的。成年中枢神经系统具有重新髓鞘的能力，产生新的少突（ OLS）。为了鉴定临床候选化合物，可以促进核髓，我们开发了一种高通量筛选（HTS）测定以鉴定促进少突胶质细胞前体细胞（OPCS）分化为OLS的化合物。使用急性解离和纯化的大鼠OPC与免疫荧光图像定量偶联，我们开发了OL分化测定。在过去30年中，在OPC培养技术中，我们已经缩放了隔离和杂化过程以产生足够的HTS。然后，我们将这些急性衍生的OPCS描述在旨在识别化合物促进OLS中的分析中的测定中的使用。我们已经验证了这种分析的分化，甲状腺激素，随后使用测定以筛选NIH临床收集文库（Lariosa-Willesham，等，2016）。

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