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首页> 外文期刊>Acta biomaterialia >Improving cell distribution on 3D additive manufactured scaffolds through engineered seeding media density and viscosity
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Improving cell distribution on 3D additive manufactured scaffolds through engineered seeding media density and viscosity

机译：通过工程播种介质密度和粘度改善3D添加剂制造支架的细胞分布

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摘要

In order to ensure the long-term in vitro and in vivo functionality of cell-seeded 3D scaffolds, an effective and reliable method to control cell seeding efficiency and distribution is crucial. Static seeding on 3D additive manufactured scaffolds made of synthetic polymers still remains challenging, as it often results in poor cell attachment, high cell sedimentation and non-uniform cell distribution, due to gravity and to the intrinsic macroporosity and surface chemical properties of the scaffolds. In this study, the biocompatible macromolecules dextran and Ficoll (Ficoll-Paque) were used for the first time as temporary supplements to alter the viscosity and density of the seeding media, respectively, and improve the static seeding output. The addition of these macromolecules drastically reduced the cell sedimentation velocities, allowing for homogeneous cell attachment to the scaffold filaments. Both dextran and Ficoll-Paque-based seeding methods supported human mesenchymal stromal cells viability and osteogenic differentiation post-seeding. Interestingly, the improved cell distribution led to increased matrix production and mineralization compared to scaffolds seeded by conventional static method. These results suggest a simple and universal method for an efficient seeding of 3D additive manufactured scaffolds, independent of their material and geometrical properties, and applicable for bone and various other tissue regeneration.
机译：为了确保细胞播种3D支架的长期体外和体内功能，控制细胞播种效率和分布的有效且可靠的方法至关重要。在合成聚合物制成的3D添加剂上静态播种仍然持挑战性，因为它往往导致细胞附着差，高细胞沉降和非均匀细胞分布，由于重力和支架的内在大孔隙度和表面化学性质。在本研究中，将生物相容性大分子葡聚糖和味精（Ficoll-Paque）首次使用，作为临时补充剂，以分别改变播种介质的粘度和密度，并改善静态播种输出。添加这些大分子大大降低了细胞沉降速度，从而允许均匀的细胞附着在支架长丝中。基于葡聚糖和粪便Paque的播种方法支持人间充质基质细胞活力和成骨分化后接种后。有趣的是，与通过常规静态法接种的支架相比，改善的细胞分布导致基质生产和矿化增加。这些结果表明了一种简单且通用的方法，用于3D添加剂制造的支架的高效播种，与其材料和几何特性无关，适用于骨骼和各种其他组织再生。

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