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首页> 外文期刊>Acta biomaterialia >Biospinning by silkworms: silk fiber matrices for tissue engineering applications.
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Biospinning by silkworms: silk fiber matrices for tissue engineering applications.

机译：用蚕生物孢子：用于组织工程应用的丝纤维矩阵。

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摘要

The mechanism of biospinning of natural silk fibers has been an open issue for decades. In this report a natural bio-polymeric matrix based on biospun silk fibers obtained from Antheraea mylitta, a wild non-mulberry tropical tasar silkworm, is put forward for potential applications. This report deals with the conformational transitions of silk fibroin during the biospinning process and its potential to support cell adherence and proliferation. The silk fibers obtained were aligned into linear, mixed or random patterns forming interconnected, macroporous three-dimensional matrices. The matrices were morphologically and functionally characterized with respect to fiber diameter, crystallinity, mechanical strength and biocompatibility using feline fibroblast cells. Drawn silk fibers showed enhanced stability to protease treatment in comparison with naturally occurring native gland fibroin protein. A viability assay suggested biocompatibility of these matrices in vitro. Fluorescence and confocal microscopy indicated normal cell attachment, spreading and proliferation on these biospun silk matrices. The results provided evidence for the use of biospun silk matrices as natural, inexpensive and alternative substrata for tissue engineering applications.
机译：天然蚕丝纤维的biospinning的机制已经有几十年的开放问题。在这份报告基于柞蚕，野无桑热带tasar蚕获得biospun丝纤维的天然生物聚合物基质中，提出了潜在的应用。本报告涉及的丝素蛋白在biospinning过程中的构象转变和其潜在的支持细胞粘附和增殖。得到的对准成直线，混合的或随机的图案形成互连的纤维丝，大孔的三维矩阵。矩阵进行形态和功能特征相对于纤维直径，结晶度，机械强度和生物相容性使用猫成纤维细胞。拉伸丝纤维显示与天然存在的天然腺素蛋白相比提高的稳定性蛋白酶处理。甲活力测定表明在体外这些矩阵的生物相容性。荧光和共焦显微镜表明已正常细胞附着，这些biospun丝矩阵扩展和增殖。结果为使用biospun丝矩阵作为用于组织工程应用的天然的，廉价的和可替换基质提供了证据。

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