• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta biomaterialia >A bilayered elastomeric scaffold for tissue engineering of small diameter vascular grafts.
【24h】

A bilayered elastomeric scaffold for tissue engineering of small diameter vascular grafts.

机译：小直径血管移植物组织工程的双层弹性支架。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A major barrier to the development of a clinically useful small diameter tissue engineered vascular graft (TEVG) is the scaffold component. Scaffold requirements include matching the mechanical and structural properties with those of native vessels and optimizing the microenvironment to foster cell integration, adhesion and growth. We have developed a small diameter, bilayered, biodegradable, elastomeric scaffold based on a synthetic, biodegradable elastomer. The scaffold incorporates a highly porous inner layer, allowing cell integration and growth, and an external, fibrous reinforcing layer deposited by electrospinning. Scaffold morphology and mechanical properties were assessed, quantified and compared with those of native vessels. Scaffolds were then seeded with adult stem cells using a rotational vacuum seeding device to obtain a TEVG, cultured under dynamic conditions for 7 days and evaluated for cellularity. The scaffold showed firm integration of the two polymeric layers with no delamination. Mechanical properties were physiologically consistent, showing anisotropy, an elastic modulus (1.4 + or - 0.4 MPa) and an ultimate tensile stress (8.3 + or - 1.7 MPa) comparable with native vessels. The compliance and suture retention forces were 4.6 + or - 0.5 x 10(-4) mmHg(-1) and 3.4 + or - 0.3N, respectively. Seeding resulted in a rapid, uniform, bulk integration of cells, with a seeding efficiency of 92 + or - 1%. The scaffolds maintained a high level of cellular density throughout dynamic culture. This approach, combining artery-like mechanical properties and a rapid and efficient cellularization, might contribute to the future clinical translation of TEVGs.
机译：临床上有用的小直径组织工程血管移植物（Tevg）的主要屏障是支架组分。支架要求包括将机械和结构性能与本机血管的匹配，并优化微环境以促进细胞集成，粘附性和生长。基于合成的可生物降解的弹性体，我们开发了小直径，双层，可生物降解，弹性体支架。支架包括高度多孔的内层，允许细胞的一体化和生长，以及通过静电纺丝沉积的外部纤维增强层。评估支架形态和机械性能，与天然血管的血管进行评估。然后使用旋转真空播种装置将支架与成人干细胞接种，得到TEVG，在动态条件下培养7天并评估细胞性。支架表明两层没有分层的两个聚合物层的整合。机械性能在生理学上一致，显示各向异性，弹性模量（1.4 +或 - 0.4MPa）和与天然容器相当的最终拉伸应力（8.3 +或-1.7MPa）。顺应性和缝合度保留力分别为4.6±0.5×10（-4）mmHg（-1）和3.4±+或 - 0.3N。播种产生了快速，均匀，散装的细胞整合，播种效率为92±1％。支架在整个动态培养方面保持高水平的细胞密度。这种方法，相当于动脉般的机械性能和快速有效的细胞化可能导致TEVGS的未来临床翻译。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号