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首页> 外文期刊>Acta biomaterialia >Plasma membrane functionalization using highly fusogenic immune activator liposomes
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Plasma membrane functionalization using highly fusogenic immune activator liposomes

机译：血浆膜官能化使用高度致致致致致致致致致致致致致致致致致致致致致致致致致致致致致致密的免疫活性剂脂质体

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摘要

Cell surface functionalization and target molecule incorporation into living cell membranes without functional damage represent major biotechnological challenges. One possible way to achieve these goals is to induce cell membrane fusion with an artificial membrane containing molecules equipped with reactive groups or ligands. In this work we developed a carrier system to incorporate lipopolysaccharide (LPS), an immune cell activating molecule from Gram-negative bacteria, into mammalian membranes. LPS is not present in untreated mammalian cells which hence are not detectable by the immune system. Here, we demonstrate the successful incorporation of LPS into fusogenic liposomes (FLs) and subsequent incorporation into mammalian plasma membranes using these FLs. Additionally, the presence of LPS in cell membranes was probed by the addition of non-activated macrophages. A high concentration of LPS in the plasma membrane of immortalized fibroblasts activated the immune cells, which in turn started to eliminate LPS-exhibiting cells. Our method for cellular membrane functionalization is a promising tool for biomedical applications and could provide the basis for specific cell targeting approaches.
机译：细胞表面官能化和靶分子掺入没有功能性损害的活细胞膜中，代表了主要的生物技术挑战。实现这些目标的一种可能方法是诱导细胞膜融合用含有配备有反应性基团或配体的人工膜的分子。在这项工作中，我们开发了一种载体系统，将脂多糖（LPS）掺入来自革兰氏阴性细菌的免疫细胞活化分子，进入哺乳动物膜。 LPS不存在于未处理的哺乳动物细胞中，因此免疫系统无法检测到。在这里，我们证明了将LPS成功地将LPS掺入富含血糖脂质体（FL）并随后使用这些液体掺入哺乳动物血浆膜中。另外，通过添加未激活的巨噬细胞探测细胞膜中LPS的存在。长化成纤维细胞的质膜中的高浓度LPS活化了免疫细胞，其又开始消除LPS表现出细胞。我们对细胞膜官能化的方法是生物医学应用的有希望的工具，可以为特定细胞靶向方法提供基础。

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  • 来源
    《Acta biomaterialia》 |2014年第3期|共9页
  • 作者

    NaumovskaE.; LudwanowskiS.; HerschN.; BraunT.; MerkelR.; HoffmannB.; CsiszárA.;

  • 作者单位

    Institute of Complex Systems ICS-7: Biomechanics Forschungszentrum Jülich GmbH 52425 Germany;

    Institute of Complex Systems ICS-7: Biomechanics Forschungszentrum Jülich GmbH 52425 Germany;

    Institute of Complex Systems ICS-7: Biomechanics Forschungszentrum Jülich GmbH 52425 Germany;

    Institute of Complex Systems ICS-7: Biomechanics Forschungszentrum Jülich GmbH 52425 Germany;

    Institute of Complex Systems ICS-7: Biomechanics Forschungszentrum Jülich GmbH 52425 Germany;

    Institute of Complex Systems ICS-7: Biomechanics Forschungszentrum Jülich GmbH 52425 Germany;

    Institute of Complex Systems ICS-7: Biomechanics Forschungszentrum Jülich GmbH 52425 Germany;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 普通生物学;
  • 关键词

    Fusogenic liposomes (FLs); Immune activation; Lipopolysaccharide (LPS); Plasma membrane functionalization;

    机译：致沉膜脂质体（FLS）;免疫激活;脂多糖（LPS）;血浆膜功能化;

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