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首页> 外文期刊>Acta biomaterialia >Poly (glycerol sebacate)-poly (epsilon-caprolactone) blend nanofibrous scaffold as intrinsic bio- and immunocompatible system for corneal repair
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Poly (glycerol sebacate)-poly (epsilon-caprolactone) blend nanofibrous scaffold as intrinsic bio- and immunocompatible system for corneal repair

机译：聚（甘油癸二酸酯） - 聚（ε-己内酮）混合纳米纤维支架，作为内在生物和免疫相容性的角膜修复系统

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摘要

A major challenge in corneal tissue engineering and lamellar corneal transplantation is to develop synthetic scaffolds able to simulate the optical and mechanical properties of the native cornea. As a carrier, the graft scaffolds should provide the basis for anchorage, repair and regeneration. Although quite a number of scaffolds have been engineered to date, they have not been able to simultaneously recapitulate chemical, mechanical, and structural properties of the corneal extracellular matrix (ECM). Here, we examined different compositions of elastomeric biodegradable poly (glycerol sebacate) (PGS)-poly (c-caprolactone) (PCL) nanofibrous scaffolds with respect to their cyto- and immunocompatibility. These scaffolds were semi-transparent with well-defined mechanical properties and direct positive effects on viability of human corneal endothelial cells (HCEC) and human conjunctival epithelial cells (HCjEC). Moreover, within 3 days HCEC established monolayers with the hexagonal morphology typical for this cell type. All PGS-PCL mixtures analyzed did not trigger effects in granulocytes, na ve and activated peripheral blood mononuclear cells (PBMCs). However, scaffolds with a higher content of PGS-PCL ratio showed the best cell organization, cyto- and immunocompatibility. Subsequently, this PGS-PCL composition could be used for further development of clinical constructs to support corneal tissue repair.
机译：角膜组织工程和层状角膜移植中的主要挑战是开发能够模拟天然角膜的光学和力学性能的合成支架。作为载体，移植支架应提供锚地，修复和再生的基础。虽然迄今为止已经设计了相当多的脚手架，但它们无法同时重新承载角膜细胞外基质（ECM）的化学，机械和结构性。在此，我们研究了不同组成的弹性体可生物降解的聚（甘油癸二酸酯）（PGS）-Pold（C-己内酯）（PCL）纳米纤维支架的纳米纤维支架，相对于它们的细胞和免疫组合性。这些支架是半透明的，具有明确定义的机械性能和对人角膜内皮细胞（HCEC）和人类结膜上皮细胞（HCJEC）的活力直接阳性作用。此外，在3天内，HCEC建立单层具有典型的该细胞类型的六边形形态学。分析的所有PGS-PCL混合物在粒细胞，Na VE和活化外周血单核细胞（PBMC）中没有触发效果。然而，具有较高含量的PGS-PCL比率的支架显示出最佳的细胞组织，细胞和免疫偶像。随后，该PGS-PCL组合物可用于进一步发展临床构建体以支持角膜组织修复。

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