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首页> 外文期刊>Acta biomaterialia >Thermoresponsive poly(glycidyl ether) brushes on gold: Surface engineering parameters and their implication for cell sheet fabrication
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Thermoresponsive poly(glycidyl ether) brushes on gold: Surface engineering parameters and their implication for cell sheet fabrication

机译：热致敬的聚（缩水甘油醚）刷子上的金色：表面工程参数及其对细胞板制造的影响

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摘要

Thermoresponsive polymer coatings, optimized for cell adhesion and thermally-triggered cell detachment, allow the fabrication of confluent cell sheets with intact extracellular matrix. However, rational design guidelines for such coatings are rare, since temperature-triggered cell adhesion and detachment from thermoresponsive surfaces are mechanistically not well understood. Herein, we investigated the impact of molecular weight (2, 9, 24 kDa), grafting density (0.04-1.4 chains nm(-2)), morphology, and roughness of well-characterized thermoresponsive poly(glycidyl ether) brushes on the cell response at 37 and 20 degrees C. NIH 3T3 mouse fibroblasts served as a model cell line for adhesion, proliferation, and cell sheet detachment. The cell response was correlated with serum protein adsorption from cell culture medium containing 10% fetal bovine serum. Intact cell sheets could be harvested from all the studied poly(glycidyl ether) coated surfaces, irrespective of the molecular weight, provided that the morphology of the coating was homogenous and the surface was fully shielded by the hydrated brush. The degree of chain overlap was estimated by the ratio of twice the polymer's Flory radius in a theta solvent to its inter chain distance, which should be located in the strongly overlapping brush regime (2 R-f/l > 1.4). In contrast, dense PNIPAM (2.5 kDa) control monolayers did not induce protein adsorption from cell culture medium at 37 degrees C and, as a result, did not allow a significant cell adhesion. These structural design parameters of functional poly(glycidyl ether) coatings on gold will contribute to future engineering of these thermoresponsive coatings on more common, cell culture relevant substrates.
机译：热响应性聚合物涂层，优化用于细胞粘附和热触发的细胞脱离，允许用完整的细胞外基质制造汇合细胞片。然而，这种涂层的合理设计指南罕见，因为温度触发的电池粘附和来自热响应表面的脱离机械地不太了解。在此，我们研究了分子量（2,9,24kDa），接枝密度（0.04-1.4链NM（-2）），形态和粗甘乙酰醚）刷细胞上的形态和粗糙度的影响在37和20℃下的响应3T3小鼠成纤维细胞用作用于粘附，增殖和细胞片脱离的模型细胞系。细胞响应与来自含有10％胎牛血清的细胞培养基的血清蛋白质吸附。完整的细胞片可以从所有研究的聚（缩水甘油醚）涂覆的表面上收获，而不管分子量如何，只要涂层的形态均匀，表面被水合刷完全屏蔽。链重叠的程度估计了Theta溶剂中的两倍于θ溶剂到其间链距离的比率，它们应位于强重叠的刷子状态（2R-F / L> 1.4）中。相反，致密肺蛋白（2.5kDa）对照单层没有在37℃下从细胞培养基中吸收蛋白质吸附，结果不允许显着的细胞粘附。金黄色官能聚（缩水甘油醚）涂层的这些结构设计参数将有助于这些热值涂层的未来工程，更常见的细胞培养相关基材。

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