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首页> 外文期刊>Acta biomaterialia >NanoTopoChip: High-throughput nanotopographical cell instruction
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NanoTopoChip: High-throughput nanotopographical cell instruction

机译：Nanotopochip：高通量纳米对文波细胞教学

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Graphical abstract Display Omitted Abstract Surface topography is able to influence cell phenotype in numerous ways and offers opportunities to manipulate cells and tissues. In this work, we develop the Nano-TopoChip and study the cell instructive effects of nanoscale topographies. A combination of deep UV projection lithography and conventional lithography was used to fabricate a library of more than 1200 different defined nanotopographies. To illustrate the cell instructive effects of nanotopography, actin-RFP labeled U2OS osteosarcoma cells were cultured and imaged on the Nano-TopoChip. Automated image analysis shows that of many cell morphological parameters, cell spreading, cell orientation and actin morphology are mostly affected by the nanotopographies. Additionally, by using modeling, the changes of cell morphological parameters could by predicted by several feature shape parameters such as lateral size and spacing. This work overcomes the technological challenges of fabricating high quality defined nanoscale features on unprecedented large surface areas of a material relevant for tissue culture such as PS and the screening system is able to infer nanotopography – cell morphological parameter relationships. Our screening platform provides opportunities to identify and study the effect of nanotopography with beneficial properties for the culture of various cell types. Statement of Significance The nanotopography of biomaterial surfaces can be modified to influence adhering cells with the aim to improve the performance of medical implants and tissue culture substrates. However, the necessary knowledge of the underlying mechanisms remains incomplete. One reason for this is the limited availability of high-resolution nanotopographies on relevant biomaterials, suitable to conduct systematic biological studies. The present study shows the fabrication of a library of nano-sized surface topographies with high fidelity. The potential of this library, called the ‘NanoTopoChip’ is shown in a proof of principle HTS study which demonstrates how cells are affected by nanotopographies. The large dataset, acquired by quantitative high-content imaging, allowed us to use predictive modeling to describe how feature dimensions affect cell morphology.
机译：图形摘要显示省略了抽象表面形貌能够以多种方式影响细胞表型，并提供操纵细胞和组织的机会。在这项工作中，我们开发了纳米TopoChip并研究了纳米级饰面的细胞有效作用。深紫外投影光刻和常规光刻的组合用于制造超过1200种不同的纳米主题的文库。为了说明纳米复印件的细胞有效效果，在纳米顶芯片上培养并成像标记U2OS骨肉瘤细胞的肌动蛋白-RFP。自动图像分析表明，许多细胞形态学参数，细胞扩散，细胞取向和肌动蛋白形态主要受纳米自行调查的影响。另外，通过使用建模，通过诸如横向尺寸和间隔的若干特征形状参数来预测细胞形态参数的变化。这项工作克服了在诸如PS和筛选系统等组织培养相关的材料的前所未有的大表面积上制造高质量定义的纳米级特征的技术挑战能够推断纳米复印机形态参数关系。我们的筛选平台提供了识别和研究纳米复印件对各种细胞类型培养物的有益特性的影响的机会。显着意义陈述可以修饰生物材料表面的纳米复印件，以影响粘附细胞，以提高医疗植入物和组织培养基材的性能。但是，对潜在机制的必要知识仍然不完整。其中一个原因是相关生物材料上的高分辨率纳米自行调查的有限可用性，适合进行系统的生物学研究。本研究表明，具有高保真性的纳米尺寸表面地形文库的制造。该图书馆的潜力称为“纳米卷上”的原理HTS研究证明，表明细胞如何受到纳米自行调查的影响。通过定量高内容成像获取的大型数据集允许我们使用预测建模来描述特征尺寸如何影响细胞形态。

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