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首页> 外文期刊>Acta biomaterialia >Alkaline phosphatase grafting on bioactive glasses and glass ceramics.
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Alkaline phosphatase grafting on bioactive glasses and glass ceramics.

机译：在生物活性玻璃和玻璃陶瓷上覆吐碱性磷酸酶接枝。

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摘要

Bone integration of orthopaedic or dental implants and regeneration of damaged bone at the surgical site are still unresolved problems in prosthetic surgery. For this reason, biomimetic surfaces (i.e. both inorganic and biological bioactive surfaces) represent a challenge for bone implantation. In this research work a hydrolase enzyme (alkaline phosphatase) was covalently grafted to inorganic bioactive glass and glass ceramic surfaces, in order to impart biological bioactivity. The functionalized samples were analysed by means of X-ray photoelectron spectroscopy in order to verify enzyme presence on the surface. Enzyme activity was measured by means of UV-visual spectroscopy after reaction with the natural substrate. Scanning electron microscopy-energy-dispersive spectroscopy observations allowed monitoring of the morphological and chemical modification of the materials during the different steps of functionalization. In vitro inorganic bioactivity was investigated by soaking samples in simulated body fluid. Enzymatic activity of the samples was tested and compared before and after soaking. Enzymatic activity of the solution was monitored at different experimental times. This study demonstrates that alkaline phosphatase could be successfully grafted onto different bioactive surfaces while maintaining its activity. Presence of the enzyme in vitro enhances the inorganic bioactivity of the materials tested.
机译：在外科或牙科植入物和手术部位的受损骨骼再生的骨骼整合仍然是假体手术的尚未解决的问题。因此，仿真表面（即无机和生物生物活性表面）代表骨植入的挑战。在该研究中，工作中，将水解酶（碱性磷酸酶）共价接枝到无机生物活性玻璃和玻璃陶瓷表面中，以赋予生物生物生物活性。通过X射线光电子能谱分析官能化样品以验证表面上的酶存在。通过与天然底物反应后通过UV视觉光谱测量酶活性。扫描电子显微镜 - 能量分散光谱观测观察允许在功能化的不同步骤中监测材料的形态学和化学改性。通过在模拟体液中浸泡样品来研究体外无机生物活性。测试样品的酶活性并在浸泡之前和之后进行比较。在不同的实验时间监测溶液的酶活性。该研究表明，碱性磷酸酶可以成功接枝到不同的生物活性表面上，同时保持其活性。体外存在酶的存在增强了所测试的材料的无机生物活性。

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