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首页> 外文期刊>Cytokine >Serum concentration of interleukin-35 and its association with tumor stages and FOXP3 gene polymorphism in patients with prostate cancer
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Serum concentration of interleukin-35 and its association with tumor stages and FOXP3 gene polymorphism in patients with prostate cancer

机译：白细胞介素-35的血清浓度及其与前列腺癌患者的肿瘤阶段和FOXP3基因多态性的关系

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IL-35 is an immunosuppressive cytokine that is largely synthesized by regulatory T (Treg) cells and may inhibit antitumor immune responses. This investigation aimed to determine the serum IL-35 concentrations and a single nucleotide polymorphism (SNP) in position of rs3761548, within the promoter region of FOXP3 gene, in patients with prostate cancer (PC). The blood specimens were obtained from 150 PC patients prior to using radiation therapy, chemo- or immunotherapy and 150 age-matched healthy men as a control group. The serum IL-35 concentrations and the pattern of genetic variation at position of rs3761548 were assessed using ELISA and polymerase chain reaction-restriction fragment length polymorphism (PCR RFLP), respectively. The mean serum IL-35 concentrations were significantly higher in PC patients when compared with healthy control group (20.01 +/- 7.03 Pg/mL vs. 11.60 +/- 2.49 Pg/mL, P 0.001). The serum IL-35 concentrations raised with progression of PC stages so that there was a significant difference between PC stages concerning the IL-35 concentrations (P 0.001). The mean serum IL-35 concentrations in patients with Gleason scores of 1-6 and Gleason scores 7-10 were significantly higher as compared with healthy controls (P 0.001). Moreover, the serum IL-35 concentrations in patients with having Gleason scores of 7-10 were significantly higher as compared with patients with Gleason scores of 1-6 (P 0.001). Evaluation of the genetic variations in position SNP rs3761548 revealed that the AA genotype and A allele were more prevalent whereas CC genotype and C allele were less prevalent in PC patients when compared with healthy men (P 0.01, P 0.001, P 0.002 and P 0.001, respectively). The AA genotype and A allele were associated with higher risk of PC incidence [OR: 2.42 (95% CI: 1.179-4.99); P 0.001 and OR: 1.732 (95% CI: 1.244 2.413); P 0.001, respectively]. The mean serum IL-35 concentrations were significantly higher in total subjects (PC patients + healthy individuals) with AA genotype and A allele than individuals with CC genotype and C allele at SNP rs3761548 (P 0.05 and P 0.01, respectively). Higher serum IL-35 concentrations observed in patients with PC that were increased with progressive tumor stages. These findings indicate that the IL-35 is possibly involve in tumor progression. Moreover, SNP rs3761548 may affect the susceptibility to PC and the serum IL-35 concentrations.
机译：IL-35是一种免疫抑制细胞因子，其主要由调节性T（Treg）细胞合成，并且可以抑制抗肿瘤免疫应答。该研究旨在确定患有前列腺癌（PC）的Foxp3基因的启动子区内Rs3761548的血清IL-35浓度和单核苷酸多态性（SNP）。在使用放射治疗，化学或免疫疗法和150岁匹配的健康男性作为对照组之前，从150名PC患者获得血液标本。使用ELISA和聚合酶链反应限制片段长度多态性（PCR RFLP）评估血清IL-35浓度和RS3761548的位置的遗传变异模式。与健康对照组相比，PC患者的平均血清IL-35浓度显着较高（20.01 +/- 7.03 pg / ml与11.60 +/- 2.49 pg / ml，P <0.001）。血清IL-35浓度随PC阶段的进展而升高，因此IL-35浓度的PC阶段存在显着差异（P <0.001）。与健康对照相比，患有Gleason分数1-6和Gleason分数7-10患者的平均血清IL-35浓度显着高（P <0.001）。此外，与Gleason评分为1-6的患者相比，患有GLEASON分数的血清IL-35浓度显着提高（P <0.001）。对位SNP RS3761548的遗传变异的评价显示，与健康男性相比，CC基因型和等位基因更普遍，而CC基因型和C等位基因在PC患者中普遍存在（P <0.01，P <0.001，P＆Lt。 0.002和P <0.001分别）。 AA基因型和等位基因与PC发生率的风险较高有关[或：2.42（95％CI：1.179-4.99）; P＆ 0.001和或：1.732（95％CI：1.244 2.413）; P＆ 0.001分别为0.001]。在SNP RS3761548（P <0.05和P <0.01，P <0.05分别为0.01分别），平均受试者（PC患者+健康个体）的血清IL-35浓度显着高于CC基因型和C等位基因的等位基因。用渐进式肿瘤阶段增加PC患者观察到的血清IL-35浓度。这些发现表明IL-35可能涉及肿瘤进展。此外，SNP RS3761548可能影响对PC和血清IL-35浓度的敏感性。

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