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首页> 外文期刊>Cytokine >Increased expression of IL-32 correlates with IFN-gamma, Th1 and Tc1 in virologically suppressed HIV-1-infected patients
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Increased expression of IL-32 correlates with IFN-gamma, Th1 and Tc1 in virologically suppressed HIV-1-infected patients

机译：IL-32的表达与IFN-GAMMA，TH1和TC1在病毒学抑制的HIV-1感染患者中的表达相关

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摘要

Following recent attention focused on IL-32 as an important component involved in the inflammatory cytokine network, we speculated that IL-32's action on IFN-gamma and IFN-gamma secreting T cell subsets may help sustain the immune activation and dysregulation found in patients with HIV-1 achieving viral suppression. To explore this hypothesis, transcript levels of IL-32 and IFN-gamma were evaluated in PBMC from 139 virologically suppressed HIV-1-infected patients and from 63 healthy individuals by Real Time RT-PCR assays. IL-32 and IFN-gamma mRNA levels were also analyzed in CD4 + T cells, CD14 + monocytes and lamina propria lymphocytes (LPL) of the gut district in a subgroup of HIV-1-infected subjects. IFN-gamma secreting CD4 + (Th1) and CD8 + (Tel) T cell subset frequencies were evaluated in LPL by multiparametric flow cytometry. Gene expression results revealed that IL-32 and IFN-gamma levels in PBMC from HIV-1-positive patients were significantly elevated compared to those from healthy donors, correlated with each other and increased with patient age. Both IL-32 and IFN-gamma genes were also more strongly expressed in CD4 + T cells than in CD14 + monocytes. By contrast, IL-32 levels in LPL were comparable to those measured in PBMC, while IFN-gamma levels were higher in PBMC than those in LPL. Negative correlations were found between IL-32 levels and the frequencies of Th1 and Tc1 subsets in gut mucosa. Collectively, our results provide the first evidence that IL-32 levels remain elevated in treated HIV-1-infected patients and correlate with IFN-gamma, Th1 and Tc1 subsets.
机译：近期关注IL-32作为炎症细胞因子网络中涉及的重要组成部分，我们推测IL-32对IFN-Gamma和IFN-Gamma分泌T细胞亚群的作用可能有助于维持患者患者的免疫激活和诱饵。 HIV-1实现病毒抑制。为了探讨该假设，通过实际时间RT-PCR测定，在PBMC中评估了IL-32和IFN-Gamma的转录水平。在HIV-1感染的受试者的亚组的CD4 + T细胞，CD14 +单核细胞和椎板上的CD4 + T细胞，CD14 +单核细胞和椎板丙氨酸淋巴细胞（LPL）中也分析了IL-32和IFN-GAMMA mRNA水平。通过多体流式细胞仪评估LPL中的IFN-Gamma分泌CD4 +（TH1）和CD8 +（Tel）T细胞子集频率。基因表达结果表明，与来自健康供体相比，HIV-1阳性患者的PBMC中的IL-32和IFN-Gamma水平显着升高，彼此相关，患者年龄增加。 IL-32和IFN-Gamma基因在CD4 + T细胞中也比CD14 +单核细胞更强烈地表达。相比之下，LPL中的IL-32水平与PBMC中测量的IL-32水平相当，而PBMC的IFN-Gamma水平比LPL中的IFN-γ水平较高。在肠粘膜中的IL-32水平和TH1和TC1亚群的频率之间发现了负相关性。统称，我们的结果提供了第一种证据，即IL-32水平在治疗的HIV-1感染患者中仍然升高，与IFN-GAMMA，TH1和TC1子集相关。

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  • 来源
    《Cytokine》 |2019年第2019期|共9页
  • 作者

    Santinelli Letizia; Statzu Maura; Pierangeli Alessandra; Frasca Federica; Bressan Alessia; Pinacchio Claudia; Nonne Chiara; Turriziani Ombretta; Antonelli Guido; dEttorre Gabriella; Scagnolari Carolina;

  • 作者单位

    Sapienza Univ Rome Ist Pasteur Italia Dept Mol Med Lab Virol Cenci Bolognetti Fdn Rome Italy;

    Sapienza Univ Rome Ist Pasteur Italia Dept Mol Med Lab Virol Cenci Bolognetti Fdn Rome Italy;

    Sapienza Univ Rome Ist Pasteur Italia Dept Mol Med Lab Virol Cenci Bolognetti Fdn Rome Italy;

    Sapienza Univ Rome Ist Pasteur Italia Dept Mol Med Lab Virol Cenci Bolognetti Fdn Rome Italy;

    Sapienza Univ Rome Ist Pasteur Italia Dept Mol Med Lab Virol Cenci Bolognetti Fdn Rome Italy;

    Sapienza Univ Rome Dept Publ Hlth &

    Infect Dis Rome Italy;

    Sapienza Univ Rome Ist Pasteur Italia Dept Mol Med Lab Virol Cenci Bolognetti Fdn Rome Italy;

    Sapienza Univ Rome Ist Pasteur Italia Dept Mol Med Lab Virol Cenci Bolognetti Fdn Rome Italy;

    Sapienza Univ Rome Ist Pasteur Italia Dept Mol Med Lab Virol Cenci Bolognetti Fdn Rome Italy;

    Sapienza Univ Rome Dept Publ Hlth &

    Infect Dis Rome Italy;

    Sapienza Univ Rome Ist Pasteur Italia Dept Mol Med Lab Virol Cenci Bolognetti Fdn Rome Italy;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    IL-32; HIV; IFN gamma; Gut; T cell subtypes;

    机译：IL-32;艾滋病毒;IFNγ;肠;T细胞亚型;

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