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首页> 外文期刊>Cytokine >Extracellular ATP activates P2X7R-NF-kappa B (p65) pathway to promote the maturation of bone marrow-derived dendritic cells of mice
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Extracellular ATP activates P2X7R-NF-kappa B (p65) pathway to promote the maturation of bone marrow-derived dendritic cells of mice

机译：细胞外ATP激活P2X7R-NF-Kappa B（P65）途径以促进小鼠的骨髓衍生的树突细胞的成熟

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摘要

The maturation state of dendritic cell (DC) plays an important role in immune activities. Previously we had found that NF-kappa B (p65) pathway could promote DC maturation and subsequent immune effects. But the upstream mechanism of this pathway was still unclear. Extracellular adenosine triphosphate (ATP) activating its receptor P2X7R has recently been considered as the fourth signal to activate T lymphocytes. Here we aimed to find out the connection between P2X7R and NF-kappa B (p65) pathway in DC maturation. Results showed that the expression of P2X7R and the intracellular ATP levels were increased along with the maturation of DC. P2X7R agonist stimulated the morphological changes of DCs into the appearance of mature DCs, and promoted the expression of NF-kappa B (p65), as well as the release of IFN-gamma and IL-12. Whereas, P2X7R inhibitor had the opposite influences. Co-immunoprecipitation assay confirmed the binding of P2X7R and NF-kappa B (p65). Our study suggested that extracellular ATP could promote DC maturation and release of inflammatory cytokines through the binding of P2X7R and NF-kappa B (p65). This is the first study to show the P2X7R-NF-kappa B (p65) pathway in DC. Interference with this pathway may be able to regulate immune responses in areas like infectious diseases, inflammation, transplantation, tumor and autoimmune diseases. In addition, intracellular ATP level could be a new indicator of the maturation state of DC.
机译：树突细胞（DC）的成熟状态在免疫活性中起重要作用。以前我们发现NF-Kappa B（P65）途径可以促进直流成熟和随后的免疫效应。但是该途径的上游机制仍然不清楚。活化其受体P2X7R的细胞外腺苷三磷酸（ATP）被认为是激活T淋巴细胞的第四信号。在这里，我们旨在在DC成熟中找到P2X7R和NF-Kappa B（P65）途径之间的连接。结果表明，P2X7R和细胞内ATP水平的表达随着DC的成熟而增加。 P2X7R激动剂刺激了DCS进入成熟DC的外观的形态变化，并促进了NF-Kappa B（P65）的表达，以及IFN-γ和IL-12的释放。虽然，P2X7R抑制剂具有相反的影响。共免疫沉淀测定证实P2X7R和NF-Kappa B的结合（P65）。我们的研究表明，通过P2X7R和NF-Kappa B的结合，细胞外ATP可以促进炎症细胞因子的直流成熟和释放（P65）。这是第一次在DC中显示P2X7R-NF-Kappa B（P65）途径的研究。干扰该途径可能能够在传染病，炎症，移植，肿瘤和自身免疫疾病等领域调节免疫应答。此外，细胞内ATP水平可以是DC成熟状态的新指标。

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  • 来源
    《Cytokine》 |2019年第2019期|共7页
  • 作者

    Yu Ying; Feng Songfu; Wei Shiyu; Zhong Yanyan; Yi Guoguo; Chen Haiyan; Liang Lifang; Chen Hui; Lu Xiaohe;

  • 作者单位

    Southern Med Univ Zhujiang Hosp Dept Ophthalmol Guangzhou 510280 Guangdong Peoples R China;

    Southern Med Univ Zhujiang Hosp Dept Ophthalmol Guangzhou 510280 Guangdong Peoples R China;

    Liuzhou Gen Hosp Dept Ophthalmol Liuzhou 545006 Peoples R China;

    Southern Med Univ Zhujiang Hosp Dept Ophthalmol Guangzhou 510280 Guangdong Peoples R China;

    Southern Med Univ Zhujiang Hosp Dept Ophthalmol Guangzhou 510280 Guangdong Peoples R China;

    Southern Med Univ Zhujiang Hosp Dept Ophthalmol Guangzhou 510280 Guangdong Peoples R China;

    Southern Med Univ Zhujiang Hosp Dept Ophthalmol Guangzhou 510280 Guangdong Peoples R China;

    Southern Med Univ Zhujiang Hosp Dept Ophthalmol Guangzhou 510280 Guangdong Peoples R China;

    Southern Med Univ Zhujiang Hosp Dept Ophthalmol Guangzhou 510280 Guangdong Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    ATP; Dendritic cell; P2X7R; NF-kappa B; Maturation;

    机译：ATP;树突细胞;P2X7R;NF-Kappa B;成熟;

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