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首页> 外文期刊>Acta Histochemica: Zeitschrift fur Histologische Topochemie >Osteoblastic differentiation potential of human amniotic fluid-derived mesenchymal stem cells in different culture conditions
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Osteoblastic differentiation potential of human amniotic fluid-derived mesenchymal stem cells in different culture conditions

机译：不同培养条件下人刚性流体衍生的间充质干细胞的骨细胞分化潜力

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Osteoporosis is a bone degenerative disease characterized by a decrease in bone strength and an alteration in the osseous micro-architecture causing an increase in the risk of fractures. These diseases usually happen in post-menopausal women and elderly men. The most common treatment involves anti-resorptive agent drugs. However, the inhibition of bone resorption alone is not adequate for recovery in patients at the severe stage of osteoporosis who already have a fracture. Therefore, the combination of utilizing osteoblast micro mimetic scaffold in cultivation with the stimulation of osteoblastic differentiations to regain bone formation is a treatment strategy of considerable interest. The aims of this current study are to investigate the osteoblastic differentiation potential of mesenchymal stem cells derived from human amniotic fluid and to compare the monolayer culture and scaffold culture conditions. The results showed the morphology of cells in human amniotic fluid as f-type, which is a typical cell shape of mesenchymal stem cells. In addition, the proliferation rate of cells in human amniotic fluid reached the highest peak after 14 days of culturing. After which time, the growth rate slowly decreased. Moreover, the positive expression of specific mesenchymal cell surface markers including CD44, CD73, CD90, and also HLA-ABC (MHC class I) were recorded. On the other hand, the negative expressions of the endothelial stem cells markers (CD31), the hematopoietic stem cells markers (CD34, 45), the amniotic stem cells markers (CD117), and also the HLA-DR (MHC class II) were also recorded. The expressions of osteoblastogenic related genes includingOCN, COL1A1,andALPwere higher in the osteogenic-induced group when compared to the control group. Interestingly, the osteoblastogenic related gene expressions that occurred under scaffold culture conditions were superior to the monolayer culture conditions. Additionally, higher ALP activity and greater calcium deposition were recorded in the extracellular matrix in the osteogenic-induced group than in the culture in the scaffold group. In summary, the mesenchymal stem cells derived from human amniotic fluid can be induced to be differentiated into osteoblastic-like cells and can promote osteoblastic differentiation using the applied scaffold.
机译：骨质疏松症是一种骨退行性疾病，其特征在于骨强度降低和骨质微观结构的改变，导致骨折的风险增加。这些疾病通常发生在绝经后妇女和老年人。最常见的治疗涉及抗复苏剂药物。然而，单独抑制骨吸收的抑制不适合于已经具有骨折的骨质疏松症的严重阶段的患者恢复。因此，利用骨细胞微模拟支架在培养中利用骨细胞分化以重新形成骨形成的培养是相当兴趣的治疗策略。本前研究的目的是研究来自人羊水的间充质干细胞的骨细胞分化电位，并比较单层培养和支架培养条件。结果表明，作为F型人羊水中细胞的形态，其是间充质干细胞的典型细胞形状。此外，14天培养后，人羊水中细胞的增殖率达到了最高峰。在此之后，生长速率慢慢减少。此外，记录了包括CD44，CD73，CD90和HLA-ABC（MHC I类）的特异性间充质细胞表面标志物的阳性表达。另一方面，内皮干细胞标记物（CD31）的阴性表达，造血干细胞标记物（CD34,45），羊酸干细胞标记物（CD117），以及HLA-DR（MHC II类）是还记录了。与对照组相比，在骨质植物诱导的组中，在骨质细胞母细胞母细胞外相关基因的表达较高。有趣的是，在支架培养条件下发生的骨纤维素母细胞植物相关基因表达优于单层培养条件。另外，在骨质发生诱导的组中的细胞外基质中记录更高的ALP活性和更大的钙沉积，而不是在支架基团中的培养物中。总之，可以诱导衍生自人羊流体的间充质干细胞分化成骨细胞状细胞，并且可以使用施加的支架促进骨细胞分化。

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