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首页> 外文期刊>Acta Histochemica: Zeitschrift fur Histologische Topochemie >Unilateral nasal obstruction alters sweet taste preference and sweet taste receptors in rat circumvallate papillae
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Unilateral nasal obstruction alters sweet taste preference and sweet taste receptors in rat circumvallate papillae

机译：单侧鼻梗阻改变甜味偏好和甜味的味道受体在甲状腺乳头上

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摘要

Nasal obstruction causes mouth breathing, and affects the growth and development of craniofacial structures, muscle function in the stomatognathic system, and the taste perceptive system. However, the detailed mechanism underlying the effects of nasal obstruction on taste perception has not been fully elucidated. In this study, we investigated this mechanism using the two-bottle taste preference test, immunohistological analysis, and quantification of the mRNA expression of taste-related molecules in the circumvallate papillae. Neonatal male Wistar rats were divided randomly into control and experimental groups. Rats in the experimental group underwent unilateral nasal obstruction by cauterization of the external nostril at the age of 8 days. Arterial oxygen saturation (SpO(2)) was recorded in awake rats using collar clip sensors. Taste preference for five basic taste solutions was evaluated. Immunohistochemical analysis and quantitative real-time polymerase chain reaction (RT-PCR) were conducted to evaluate the expressions of taste-related molecules in the taste cells of the circumvallate papillae. Body weights were similar between the two groups throughout the experimental period. The SpO(2) in the 7- to 12-week-old rats in the experimental group was significantly lower than that in the age matched rats in the control group. In the two-bottle taste preference test, the sensitivities to sweet taste decreased in the experimental group. The mRNA expression of T1R2, T1R3, alpha-gustducin, and PLC beta 2 was significantly lower in the experimental group than in the control group as determined by quantitative RT-PCR, and the immunohistochemical staining for a-gustducin and PLC beta 2 was less prominent. These findings suggest that nasal obstruction may affect sweet taste perception via the reduced expression of taste-related molecules in the taste cells in rat circumvallate papillae.
机译：鼻梗阻导致口感呼吸，并影响颅面结构的生长和发展，气象系统中的肌肉功能，以及味道感知系统。然而，鼻塞对鼻梗阻对味觉感知的影响的详细机制尚未完全阐明。在这项研究中，我们使用双瓶口味偏好试验，免疫组织学分析和定量味道乳头中味道相关分子mRNA表达的定量来研究该机制。新生儿雄性Wistar大鼠随机分为对照和实验组。实验组大鼠通过在8天的外部鼻孔的烧灼后接受单侧鼻塞。使用套环夹传感器在清醒大鼠中记录动脉氧饱和度（SPO（2））。评估五种基本味道溶液的味道偏好。进行免疫组织化学分析和定量实时聚合酶链反应（RT-PCR）以评估味道乳头的味道细胞中味道相关分子的表达。在整个实验期间两组之间的体重相似。实验组7至12周龄大鼠的SPO（2）显着低于对照组的年龄匹配大鼠。在双瓶口味偏好试验中，实验组中对甜味的敏感性降低。实验组中T1R2，T1R3，α-甘蓝和PLCβ2的mRNA表达显着低于通过定量RT-PCR测定的对照组，并且对α-甘蓝和PLCβ2的免疫组织化学染色较小著名的。这些发现表明，鼻塞可能会通过降低大鼠味道乳头的味道细胞中的味觉相关分子的表达减少来影响甜味感知。

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