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Identification of milk whey protein biomarkers of early pregnancy status in dairy cattle

机译：乳品牛早期妊娠状况的乳乳清蛋白生物标志物的鉴定

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摘要

Current bovine pregnancy detection methods include rectal palpation, ultrasound scanning, PregnancyAssociated Glycoprotein ELISA assays, Pregnancy Specific Protein B ELISA assays, progesterone immunoassays, in-lineprogesterone testing and visual observation of oestrus (often with the use of aids to detection; e.g. tail paint/heat detectionpads) (Balhara, et al., 2013). However, these assays suffer from limited sensitivity and results from these pregnancy testmethods are not reliable until at least day 28 to 35 post artificial insemination (AI). Therefore, there is a need for thedevelopment of an early, reliable, pregnancy test, which is valid on or before day 21 of pregnancy, and would allowproducers the opportunity to rebreed at the next oestrus. Ideally, this pregnancy test would utilise milk as the preferredanalyte, as a sample can be easily obtained during routine milking and consequently, will not impose any additional stresson the cows.
机译：目前的牛妊娠检测方法包括直肠触诊，超声扫描，怀孕糖蛋白酶ELISA测定，妊娠特异性蛋白B ELISA测定，孕酮免疫测定，内孕酮检测和发作的视觉观察（通常使用艾滋病检测;例如尾部涂料/ 热量检测板）（Balhara，et al。，2013）。 然而，这些测定患有有限的敏感性，并且这些妊娠试验方法的结果不可靠，直到人工授精至少第28至35天至35次。 因此，需要早期，可靠，妊娠试验，在怀孕第21天或之前有效，并且允许生产者在下一个令人讨厌的情况下有机会重新释放。 理想情况下，这种妊娠试验将利用牛奶作为首选体内，因为在常规挤奶期间可以容易地获得样品，因此不会施加任何额外的应力奶牛。

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  • 来源
    《Advances in Animal Biosciences》 |2018年第1期|共1页
  • 作者

    D Johnston; I Malo Estepa; H A Ebhardt; M A Crowe; M G Diskin;

  • 作者单位

    Animal Bioscience Research Department Animal &

    Grassland Research and Innovation Centre Teagasc Grange Dunsany Co. Meath Ireland;

    School of Veterinary Medicine Veterinary Sciences Centre University College Dublin Belfield Dublin 4 Ireland;

    Systems Biology Ireland University College Dublin Belfield Dublin 4 Ireland;

    Animal Bioscience Research Department Animal &

    Grassland Research and Innovation Centre Teagasc Mellows Campus Athenry Co. Galway Ireland;

    School of Veterinary Medicine Veterinary Sciences Centre University College Dublin Belfield Dublin 4 Ireland;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 动物学;
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