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首页> 外文期刊>ACS Sustainable Chemistry & Engineering >Comparative Study of in Situ and ex Situ Enzymatic Hydrolysis of Milk Protein and Separation of Bioactive Peptides in an Electromembrane Reactor
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Comparative Study of in Situ and ex Situ Enzymatic Hydrolysis of Milk Protein and Separation of Bioactive Peptides in an Electromembrane Reactor

机译：牛奶蛋白原位和原位酶水解的比较研究及电磁蛋白反应器中的生物活性肽分离

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摘要

Tryptic hydrolysis of whey protein isolate was performed simultaneously during (in situ) and before (ex situ) fractionation by electrodialysis with ultrafiltration membrane (EDUF) to obtain bioactive peptides. Peptide migration to anionic (A(RC)(-)) and cationic (C-RC(+)) peptide recovery compartments was strongly dependent on the digestion strategy used. Indeed, peptide migration to the A(RC)(-) was observed to be higher with in situ digestion while peptide migration to the C-RC(+) was higher in an ex situ digestion: a final peptide concentration of 103.10 +/- 2.76 mu g/mL was found in the C-RC(+) (ex situ) while it was 49.65 +/- 6.13 mu g/mL in the A(RC)(-) (in situ). HPLC-MS studies showed 23 major peaks that were generated by tryptic digestion of whey protein isolate. Seven of these peptides migrated to the A(RC)(-) while nine and eight peptides migrated to the C-RC(+) for ex situ and in situ digestions, respectively. Among them, different antihypertensive, antimicrobial, and hypocholesterolemic peptides were recovered depending on the recovery compartment such as IDALNENK and VYVEELKPTPEGDLEILLQK in A(RC)(-) and ALPMHIR, TKIPAVFK, VLVLDTDYKK, and VAGTWY in C-RC(+) regardless of the mode of enzymatic hydrolysis.
机译：在用超滤膜（EDUF）的电渗析（EX原位）和之前（EX原位）分馏同时进行乳清蛋白分离物的胰蛋白水解，以获得生物活性肽。肽迁移至阴离子（A（RC）（ - ））和阳离子（C-RC（+））肽回收室强烈依赖于所用的消化策略。实际上，观察到肽迁移到A（RC）（ - ）的原位消化以较高的肽迁移到蛋白质消化中较高：最终肽浓度为103.10 +/-在C-RC（+）（EX原位）中发现2.76μg/ ml，而A（RC）（ - ）（原位）中为49.65 +/-6.13μg/ ml。 HPLC-MS研究表明，由乳清蛋白分离物的胰蛋白酶消化产生的23个主要峰。这些肽中的七种迁移到A（RC）（ - ）（ - ），而九个和8个肽分别迁移到Ex原位的C-RC（+）和原位消化。其中，取决于恢复室，如idalneNk和Vyveelkptpeglearqk在A（RC）（ - ）和Alpmhir，Tkipavfk，VLVLDTDYKK和C-RC（+）中的Vytwy（+）中，恢复不同的抗高血压，抗菌药物和脱胆固醇肽肽，而无论如何酶水解模式。

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    Suwal Shyam; Rozoy Elodie; Manenda Mander; Doyen Main; Bazinet Laurent;

  • 作者单位

    Univ Laval Dept Food Sci Dairy Res Ctr STELA Inst Nutr &

    Funct Foods INAF Lab Food Proc &

    Elec 2425 Rue Agr Quebec City PQ G1V 0A6 Canada;

    Univ Laval Dept Food Sci Dairy Res Ctr STELA Inst Nutr &

    Funct Foods INAF Lab Food Proc &

    Elec 2425 Rue Agr Quebec City PQ G1V 0A6 Canada;

    Univ Laval Dept Food Sci Dairy Res Ctr STELA Inst Nutr &

    Funct Foods INAF Lab Food Proc &

    Elec 2425 Rue Agr Quebec City PQ G1V 0A6 Canada;

    Univ Laval Dept Food Sci Dairy Res Ctr STELA Inst Nutr &

    Funct Foods INAF Lab Food Proc &

    Elec 2425 Rue Agr Quebec City PQ G1V 0A6 Canada;

    Univ Laval Dept Food Sci Dairy Res Ctr STELA Inst Nutr &

    Funct Foods INAF Lab Food Proc &

    Elec 2425 Rue Agr Quebec City PQ G1V 0A6 Canada;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学工业;
  • 关键词

    In situ; Ex situ; Enzymatic hydrolysis; Bioactive peptides separation; Electromembrane reactor;

    机译：原位;ex原位;酶水解;生物活性肽分离;电磁蛋白反应器;

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