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首页> 外文期刊>ACS Sustainable Chemistry & Engineering >Expanding the Potential Role of Deep Eutectic Solvents toward Facilitating the Structural and Thermal Stability of alpha-Chymotrypsin
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Expanding the Potential Role of Deep Eutectic Solvents toward Facilitating the Structural and Thermal Stability of alpha-Chymotrypsin

机译：扩大深度共晶溶剂对促进α-chymotrypsin的结构和热稳定性的潜在作用

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Since the introduction of deep eutectic solvents (DESs), numerous reports have surfaced describing its tunable properties and environmentally benign nature. Due to such favorable properties of DESs, they have found a wide variety of applications. Moreover, in order to harness the potential of proteins in numerous industries, there is an emergent need to find a suitable cosolvent that is biocompatible with protein and is also environmentally safe. In this context, this work presents a systematic evaluation of effect of two deep eutectic solvents (DESs), namely, choline chloride-urea (ChCl-urea) and choline chloride-glycerol (ChCl-gly) on the structural and thermal stability along with activity of enzyme alpha-chymotrypsin (CT) using circular dichroism (CD), UV-visible, steady state, and thermal fluorescence spectroscopy. It was observed that the presence of DESs does lead to enhancement in the thermal stability of CT along with the preservation of activity. The enzymatic activity was well maintained in both the DESs, and the deleterious effect of urea was overcome by ChCl-urea on the enzyme. Also, desirable results were observed for ChCl-urea, despite having urea as one of its major components. Thus, the negative outcome of urea was overpowered by the combination of ChCl and urea. Furthermore, all the biomolecular studies were also performed with the individual constituents of DESs. It was found that the effect imparted by both the ChCl-based DESs on CT is by the virtue of DES itself rather than its individual constituent. Overall, both the DESs can be described as potential biocompatible, sustainable, and promising cosolvents for CT with enhanced structural and thermal stability along with preservation of its activity.
机译：由于引入深层共晶溶剂（DESS），因此许多报告已经浮出水面描述了其可调性和环境良性性质。由于Dess的这种有利性，他们已经发现了各种各样的应用。此外，为了利用众多产业中蛋白质的潜力，出现的需要找到一种与蛋白质生物相容的合适的共存溶剂，也是环保的。在这种情况下，该工作介绍了两个深度共晶溶剂（DESS），即胆碱氯化脲 - 尿素（ChCl-urea）和胆碱 - 甘油（ChCl-Gly）以及结构和热稳定性的影响的系统评价使用圆形二色性（CD），UV可见，稳态和热荧光光谱法的酶α-Chymotrypsin（CT）的活性。观察到DES的存在确实导致CT的热稳定性以及活性的保存。酶活性在DES中保持良好，尿素的有害效果被酶对酶进行克服。此外，尽管将尿素作为其主要成分之一，但仍观察到CHCl-urea的理想结果。因此，尿素的负面结果通过CHCL和尿素的组合来压迫。此外，所有的生物分子研究也用Dess的个体成分进行。发现基于CHCL的DES赋予CT上的效果是通过DES本身而不是其个体成分的效果。总的来说，DESS都可以被描述为具有增强结构和热稳定性的CT的潜在的生物相容性，可持续性和有前途的助理，以及保存其活性。

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