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首页> 外文期刊>ACS Sustainable Chemistry & Engineering >Sensitive Sulfide Monitoring in Live Cells by Dark-Field Microscopy Based on the Formation of Ag2S on Au@AgI Core-Shell Nanoparticles
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Sensitive Sulfide Monitoring in Live Cells by Dark-Field Microscopy Based on the Formation of Ag2S on Au@AgI Core-Shell Nanoparticles

机译：基于AG2S在Au @ AGI核心 - 壳纳米粒子的形成暗场显微镜敏感硫化物监测

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Hydrogen sulfide (H2S) is the third endogenous gas in mammals that plays an important role in understanding human physiological and pathological processes. However, it remains difficult to measure H2S in living biological specimens due to interference of other biothiols (GSH, Cys, Hcy, and thiol-containing proteins) as well as low concentrations of H2S (as low as sub-nM). Here, we present Au@AgI core-shell plasmonic nanoparticles (PNPs) as highly sensitive probes to acquire sulfide rapid monitoring in biological environments. When the Au@AgI PNPs are exposed to sulfide, the AgI transforms into Ag2S, leading a change of local surface plasmon resonance (LSPR), thereby resulting in a color and light intensity change at the single nanoparticle level which can be monitored by dark-field microscopy (DFM). This strategy has an ultralow limit of detection (LOD) of 33 pM and great antiinterference ability for sulfide detection in biological environments. This method was successfully used for highly sensitive sulfide mapping in live cells and to record the changes of H2S levels in different brain regions of rats during acute cerebral ischemia, validating that this method suitable for trace sulfide sensing in biological environments. We anticipate that this sulfide sensor has potential applications for studying complex neurochemical changes.
机译：硫化氢（H2S）是哺乳动物中的第三内源气体，在理解人类生理和病理过程方面发挥着重要作用。然而，由于其他生物醇（GSH，Cys，Hcy和硫醇的蛋白质）以及低浓度的H 2 S（低至Sub-NM），仍然难以测量生物生物标本中的H2S。在这里，我们将Au @ Agi核心 - 壳等级纳米粒子（PNPS）作为高敏感性探针，以获得生物环境中的硫化物快速监测。当Au @ AGI PNP暴露于硫化物时，AGI转化为Ag2s，导致局部表面等离子体共振（LSPR）的变化，从而导致单个纳米颗粒水平的颜色和光强度变化，可以通过暗 - 现场显微镜（DFM）。该策略具有33:33下午33点的超级检测（LOD），并且在生物环境中硫化物检测的抗干扰能力很大。该方法已成功用于活细胞中的高敏感性硫化物测绘，并在急性脑缺血期间记录大鼠不同脑区中H2S水平的变化，验证该方法适用于生物环境中痕量硫化物感应。我们预期该硫化物传感器具有用于研究复杂神经化学变化的潜在应用。

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