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首页> 外文期刊>ACS Sustainable Chemistry & Engineering >High-Voltage Pulsed Electric Field Preprocessing Enhances Extraction of Starch, Proteins, and Ash from Marine Macroalgae Ulva ohnoi
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High-Voltage Pulsed Electric Field Preprocessing Enhances Extraction of Starch, Proteins, and Ash from Marine Macroalgae Ulva ohnoi

机译：高压脉冲电场预处理增强了淀粉，蛋白质和灰烬的提取，来自海洋Macroalgae Ulva Ohnoi

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摘要

Marine macroalgae are an attractive source for biorefineries as an alternative to terrestrial crops, and new, sustainable macroalgae biomass fractionation methods are needed. One of the least investigated macroalgae-derived products is starch. In this work, we report on a device and a protocol for pretreatment for starch extraction from a green macroalga Ulva ohnoi (U. ohnoi) with an emerging, non-thermal, and environmental friendly technology-pulsed electric field (PEF). Using the custom-made insulated gate bipolar transistor-pulsed generator combined with a gravitation press-electrode device, we show that 200 pulses with a field strength of 1 kV cm(-1), pulse duration of 50 mu s, and pulse repetition rate of 3 Hz concentrate the starch in the U. ohnoi biomass by 59.38% by removing the salts, proteins, and other small molecules. The starch extraction yield from the PEF-pretreated biomass is 59.54 +/- 1.34%, compared to 52.31 +/- 3.21% from untreated biomass. In addition, PEF combined with pressing increased the coextracted macroalgae protein by more than 4 times and ash by 1.5 times in comparison with pressing alone. These results indicate the potential of PEF pretreatment for challenging macroalgae biomass fractionation in the sustainable marine biorefinery.
机译：Marine Macroalgae是对生物猎物的一种有吸引力的来源，作为陆地作物的替代品，而且需要新的可持续的大型宏观物质分馏方法。最多研究的大草种衍生产品是淀粉。在这项工作中，我们报告了一种装置和一种用于从绿色大型宏峰ULVA OHNOI（U. OHNOI）的预处理的预处理方案，具有新兴，非热和环保技术脉冲电场（PEF）。使用定制的绝缘栅双极晶体管脉冲发电机与重力压力机器件组合，我们显示200个脉冲，场强度为1 kV cm（-1），脉冲持续时间，脉冲持续时间和脉冲重复率通过除去盐，蛋白质和其他小分子，3Hz将淀粉浓缩59.38％的淀粉。来自PEF预处理生物质的淀粉提取产率为59.54 +/- 1.34％，而未治疗生物质的52.31 +/- 3.21％。此外，与单独的单独压制相比，PEF与压制增加了4倍以上的4次和灰分。这些结果表明了PEF预处理的潜力，用于挑战可持续海洋生物遗料中的挑战大草原生物质分馏。

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