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首页> 外文期刊>Crystallography reports >Preliminary small-angle X-ray scattering and X-ray diffraction studies of the BTB domain of lola protein from Drosophila melanogaster
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Preliminary small-angle X-ray scattering and X-ray diffraction studies of the BTB domain of lola protein from Drosophila melanogaster

机译:来自果蝇黑素糖蛋白酶的Lola蛋白BTB域的初步小角X射线散射和X射线衍射研究

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摘要

The Drosophila genome has several dozens of transcription factors (TTK group) containing D'D cent D' domains assembled into octamers. The LOLA protein belongs to this family. The purification, crystallization, and preliminary X-ray diffraction and small-angle X-ray scattering (SAXS) studies of the BTB domain of this protein are reported. The crystallization conditions were found by the vapor-diffusion technique. A very low diffraction resolution (8.7 resolution) of the crystals was insufficient for the determination of the threedimensional structure of the BTB domain. The SAXS study demonstrated that the BTB domain of the LOLA protein exists as an octamer in solution.
机译:果蝇基因组有几十个转录因子(TTK组),其含有组装成八羟胺的d'd厘米d'结构域。 萝拉蛋白质属于这个家庭。 报道了该蛋白质的BTB结构域的纯化,结晶和初步X射线衍射和小角X射线散射(SAXS)研究。 通过气相扩散技术发现结晶条件。 晶体的非常低的衍射分辨率(8.7分辨率)不足以确定BTB结构域的三维结构。 SAXS研究表明,Lola蛋白的BTB结构域作为溶液中的八次蛋白。

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  • 来源
    《Crystallography reports》 |2017年第6期|共4页
  • 作者单位

    Russian Acad Sci Fed Res Ctr Fundamentals Biotechnol Moscow 119071 Russia;

    Russian Acad Sci Fed Res Ctr Fundamentals Biotechnol Moscow 119071 Russia;

    Natl Res Ctr Kurchatov Inst Moscow 123098 Russia;

    Russian Acad Sci Inst Gene Biol Moscow 119334 Russia;

    Natl Res Ctr Kurchatov Inst Moscow 123098 Russia;

    Russian Acad Sci Fed Res Ctr Fundamentals Biotechnol Moscow 119071 Russia;

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  • 正文语种 eng
  • 中图分类 晶体学;
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