Crystallization and preliminary X-ray diffraction studies of Drosophila melanogaster G alpha o-subunit of heterotrimeric G protein in complex with the RGS domain of CG5036

Tishchenko Svetlana; Gabdulkhakov Azat; Tin Uliana; Kostareva Olga; Lin Chen; Katanaev Vladimir L.

首页> 外文期刊>Acta crystallographica, Section F. Structural biology and crystallization communications >Crystallization and preliminary X-ray diffraction studies of Drosophila melanogaster G alpha o-subunit of heterotrimeric G protein in complex with the RGS domain of CG5036

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Crystallization and preliminary X-ray diffraction studies of Drosophila melanogaster G alpha o-subunit of heterotrimeric G protein in complex with the RGS domain of CG5036

机译：与CG5036的RGS结构域复合的异三聚体G蛋白的果蝇果蝇G alpha o-亚基的结晶和初步X射线衍射研究

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Regulator of G-protein signalling (RGS) proteins negatively regulate heterotrimeric G-protein signalling through their conserved RGS domains. RGS domains act as GTPase-activating proteins, accelerating the GTP hydrolysis rate of the activated form of G alpha-subunits. Although omnipresent in eukaryotes, RGS proteins have not been adequately analysed in non-mammalian organisms. The Drosophila melanogaster G alpha o-subunit and the RGS domain of its interacting partner CG5036 have been overproduced and purified; the crystallization of the complex of the two proteins using PEG 4000 as a crystallizing agent and preliminary X-ray crystallographic analysis are reported. Diffraction data were collected to 2.0 angstrom resolution using a synchrotron-radiation source.

机译：G蛋白信号转导（RGS）蛋白的调节剂通过其保守的RGS域负调控异源三聚体G蛋白信号转导。 RGS结构域充当GTPase激活蛋白，可加快Gα亚基激活形式的GTP水解速度。尽管真核生物中普遍存在RGS蛋白，但尚未在非哺乳动物生物中对其进行充分的分析。果蝇果蝇Gαo亚基和它的相互作用伙伴CG5036的RGS结构域已经过生产和纯化。报道了使用PEG 4000作为结晶剂对两种蛋白质的复合物进行结晶和初步X射线晶体学分析的报道。使用同步辐射源将衍射数据收集到2.0埃分辨率。

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《Acta crystallographica, Section F. Structural biology and crystallization communications》 |2013年第1期|共4页
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Tishchenko Svetlana; Gabdulkhakov Azat; Tin Uliana; Kostareva Olga; Lin Chen; Katanaev Vladimir L.;
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1. Crystallization and preliminary X-ray diffraction studies of Drosophila melanogaster G alpha o-subunit of heterotrimeric G protein in complex with the RGS domain of CG5036 [J] . Tishchenko Svetlana, Gabdulkhakov Azat, Tin Uliana, Acta crystallographica, Section F. Structural biology and crystallization communications . 2013,第1期

机译：与CG5036的RGS结构域复合的异三聚体G蛋白的果蝇果蝇G alpha o-亚基的结晶和初步X射线衍射研究
2. Preliminary small-angle X-ray scattering and X-ray diffraction studies of the BTB domain of lola protein from Drosophila melanogaster [J] . Boyko K. M., Nikolaeva A. Yu., Kachalova G. S., Crystallography reports . 2017,第6期

机译：来自果蝇黑素糖蛋白酶的Lola蛋白BTB域的初步小角X射线散射和X射线衍射研究
3. Structural investigation of the ligand binding domain of the zebrafish VDR in complexes with 1alpha,25(OH)2D3 and Gemini: purification, crystallization and preliminary X-ray diffraction analysis. [J] . Ciesielski F, Rochel N, Mitschler A, The Journal of Steroid Biochemistry and Molecular Biology . 2004,第1a5期

机译：斑马鱼VDR与1alpha，25（OH）2D3和Gemini配合物的配体结合结构域的结构研究：纯化，结晶和初步X射线衍射分析。
4. Measurement of Receptor-Binding Activity of Non-Steroidal Ecdysone Agonists Using in vitro Expressed Receptor Proteins (EcR/USP Complex) of Chilo suppressalis and Drosophila melanogaster [C] . Chieka Minakuchi, Yoshiaki Nakagawa, Manabu Kamimura, Pan-Pacific Conference on Pesticide Science . 2005

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5. Stringent starvation protein A: Expression, purification, characterization, and preliminary x-ray diffraction studies [D] . Andrykovitch, Michelle 2002

机译：严格的饥饿蛋白A：表达，纯化，表征和初步X射线衍射研究
6. Crystallization and preliminary X-ray diffraction studies of Drosophila melanogaster Gαo-subunit of heterotrimeric G protein in complex with the RGS domain of CG5036 [O] . Svetlana Tishchenko, Azat Gabdulkhakov, Uliana Tin, 2013

机译：与CG5036的RGS结构域复合的异三聚体G蛋白的果蝇果蝇Gαo亚基的结晶和初步X射线衍射研究
7. Crystallization and preliminary X-ray diffraction studies of FHA domains of Dun1 and Rad53 protein kinases [O] . Blanchard H., Fontes R. M, Hammet A., 2001

机译：Dun1和Rad53蛋白激酶FHA结构域的结晶和初步X射线衍射研究

Crystallization and preliminary X-ray diffraction studies of Drosophila melanogaster G alpha o-subunit of heterotrimeric G protein in complex with the RGS domain of CG5036

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