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Investigations into Protein Crystallization in the Presence of a Strong Magnetic Field

机译：强磁场作用下蛋白质结晶的研究

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摘要

A new strategy is proposed for batch crystallization of proteins in solution-growth or gel-growth by using the batch method inside capillary tubes applying magnetic fields. Four proteins with differing proportions of alpha-helices and beta-sheets and crystallized in five different crystallographic space groups are studied, allowing an analysis of the anisotropy of the diamagnetic susceptibility of the peptide bond its well as the polarity of the space groups in the presence of a strong magnetic field of 11.75 T. The crystal quality is shown to be improved by using a strong magnetic field to orient protein molecules, and gel-growth (high concentrations of agar) to control the transport phenomena as well as crystal growth. Sonic advantages to increase the crystal quality for crystals from marginal conditions for X-ray diffraction, and disadvantages of the use of solution- and gel-growth (low concentration of agar) in magnetic Fields, and their plausible applications to high resolution X-ray crystallography are discussed.
机译：提出了一种新的策略，可以通过在毛细管内部施加磁场的分批方法，使溶液增长或凝胶增长中的蛋白质分批结晶。研究了四种具有不同比例的α-螺旋和β-折叠的蛋白质，并在五个不同的晶体空间群中进行了结晶，从而可以分析肽键的抗磁化率的各向异性以及存在时空间群的极性11.75 T的强磁场。通过使用强磁场来定向蛋白质分子和凝胶生长（高浓度的琼脂）来控制转运现象和晶体生长，可以改善晶体质量。声波的优势是从X射线衍射的极限条件提高晶体的晶体质量，在磁场中使用溶液和凝胶生长（琼脂浓度低）的缺点，以及它们在高分辨率X射线中的合理应用晶体学讨论。

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