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首页> 外文期刊>Crystal growth & design >Tyrosine microcrystals produced by digestion of proteins with pancreatic enzymes
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Tyrosine microcrystals produced by digestion of proteins with pancreatic enzymes

机译：通过胰酶消化蛋白质产生的酪氨酸微晶

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If serum albumin from several animals, including humans, at concentrations greater than about 60 mg/mL is combined with an aqueous extract of porcine or human pancreas, then massive amounts of microcrystals develop. Both raw blood serum, as well as a range of concentrated, pure proteins, when combined with the extract produced identical crystals. Morphologies of crystals and their aggregates did, however, show some dependence on the starting protein material. Crystals could be dissolved by boiling in water and recrystallized. By X-ray diffraction we showed the crystals to be l-tyrosine crystals, presumably a degradation product of the starting proteins. Amino acid analysis, supported by mass spectrometry, of even well washed crystals, however, consistently showed them to be composed of 20% to 30% of other amino acids. An atomic force microscopy investigation of the crystals revealed that the crystal surfaces were persistently coated with spherical particles about 3 nm in diameter. The particles are apparently micellar arrangements of oligopeptides. Some implications of our results with regard the appearance of tyrosine crystals in human tissues under pathological conditions, in tumors, and in natural products such as foods are discussed.
机译：如果将浓度大于约60 mg / mL的几种动物（包括人）的血清白蛋白与猪或人胰腺的水提物混合，则会形成大量的微晶。当与提取物结合使用时，原始血清以及一系列浓缩的纯蛋白质都会产生相同的晶体。但是，晶体及其聚集体的形态确实显示出对起始蛋白质材料的某种依赖性。晶体可以通过在水中煮沸而溶解并重结晶。通过X射线衍射，我们显示该晶体为1-酪氨酸晶体，大概是起始蛋白质的降解产物。质谱分析还对洗净得很好的晶体进行了氨基酸分析，但始终如一地表明它们由20％至30％的其他氨基酸组成。原子力显微镜对晶体的研究表明，晶体表面持续被直径约3 nm的球形颗粒覆盖。该颗粒显然是寡肽的胶束排列。关于酪氨酸晶体在病理条件下在人体组织中，在肿瘤中以及在天然产物（例如食物）中的出现，我们的结果有一些启示。

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