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首页> 外文期刊>Alcohol >Alpha-ketoglutarate reduces ethanol toxicity in Drosophila melanogaster by enhancing alcohol dehydrogenase activity and antioxidant capacity
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Alpha-ketoglutarate reduces ethanol toxicity in Drosophila melanogaster by enhancing alcohol dehydrogenase activity and antioxidant capacity

机译：Alpha-Ketoglutarate通过提高酒精脱氢酶活性和抗氧化能力，减少果蝇乙醇毒性的乙醇毒性

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摘要

Ethanol at low concentrations (<4%) can serve as a food source for fruit fly Drosophila melanogaster, whereas at higher concentrations it may be toxic. In this work, protective effects of dietary alpha-ketoglutarate (AKG) against ethanol toxicity were studied. Food supplementation with 10-mM AKG alleviated toxic effects of 8% ethanol added to food, and improved fly development. Two-day-old adult flies, reared on diet containing both AKG and ethanol, possessed higher alcohol dehydrogenase (ADH) activity as compared with those reared on control diet or diet with ethanol only. Native gel electrophoresis data suggested that this combination diet might promote post-translational modifications of ADH protein with the formation of a highly active ADH form. The ethanol-containing diet led to significantly higher levels of triacylglycerides stored in adult flies, and this parameter was not altered by AKG supplement. The influence of diet on antioxidant defenses was also assessed. In ethanol-fed flies, catalase activity was higher in males and the levels of low molecular mass thiols were unchanged in both sexes compared to control values. Feeding on a mixture of AKG and ethanol did not affect catalase activity but caused a higher level of low molecular mass thiols compared to ethanol-fed flies. It can be concluded that both a stimulation of some components of antioxidant defense and the increase in ADH activity may be responsible for the protective effects of AKG diet supplementation in combination with ethanol. The results suggest that AKG might be useful as a treatment option to neutralize toxic effects of excessive ethanol intake and to improve the physiological state of D. melanogaster and other animals, potentially including humans. (C) 2016 Elsevier Inc. All rights reserved.
机译：低浓度（<4％）的乙醇可以作为果蝇果蝇的食物来源，而在较高的浓度下，它可能是有毒的。在这项工作中，研究了膳食Alpha-Ketoglutarate（Akg）对乙醇毒性的保护作用。用10毫米AKG的食品补充剂缓解了8％乙醇的毒性效果，并改善了飞行发育。两天历史的成年苍蝇，饲养含有Akg和乙醇的饮食，与饲养的乙醇饲养的人相比，具有较高的酒精脱氢酶（ADH）活性。本地凝胶电泳数据表明，这种组合饮食可能促进ADH蛋白的翻译后修饰，形成高活度的ADH形式。含乙醇的饮食导致储存在成人苍蝇中的三酰基甘油水平显着较高，并且该参数不会通过Akg补充剂改变。还评估了饮食对抗氧化防御的影响。在乙醇喂的苍蝇中，与对照值相比，雄性的母体活性较高，低分子量硫醇的水平不变。饲喂Akg和乙醇的混合物不影响过氧化氢酶活性，但与乙醇喂食苍蝇相比，导致更高水平的低分子质量硫醇。可以得出结论，刺激抗氧化防御和ADH活性的增加的刺激可能是AKG饮食补充与乙醇组合的保护作用。结果表明，AKG可用作治疗选项以中和过量乙醇摄入的毒性作用，并改善D. melanogaster和其他动物的生理状态，可能包括人类。 （c）2016年Elsevier Inc.保留所有权利。

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