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首页> 外文期刊>Acta Poloniae Pharmaceutica: Durg Research >DETERMINATION OF FLUCONAZOLE IN HUMAN PLASMA BY REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
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DETERMINATION OF FLUCONAZOLE IN HUMAN PLASMA BY REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

机译：反相高效液相色谱法测定人血浆中的氟康唑。

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摘要

A rapid, simple and reproducible high performance liquid chromatographic method was developed and validated for determination of fluconazole in human plasma. The separation was performed on MZ C8 column (125 x 4 mm, 5 pm) using acetonitrile - potassium dihydrogen phosphate buffer (15 : 85, v/v), pH 3.0, as the mobile phase at a flow rate of 1.5 mL/min. The wavelength was set at 261 nun. The assay enables the measurement of fluconazole for therapeutic drug monitoring with a minimum quantification limit of 20 ng/mL. The method involves simple, protein precipitation procedure and analytical recovery was complete. The calibration curve was linear over the concentration range 0.1-4 mu g/mL. The coefficients of variation for inter-day and intra- day assay were found to be less than 10%.
机译：建立了一种快速，简单，可重现的高效液相色谱方法，并经过验证可用于测定人血浆中的氟康唑。在MZ C8色谱柱（125 x 4 mm，5 pm）上使用乙腈-磷酸二氢钾缓冲液（15：85，v / v），pH 3.0，流动相以1.5 mL / min的流速进行分离。波长设置为261 nun。该测定法能够对氟康唑进行测量，以监测治疗药物，最低定量限为20 ng / mL。该方法涉及简单的蛋白质沉淀过程，分析回收已完成。校准曲线在0.1-4μg / mL的浓度范围内是线性的。发现日间和日内分析的变异系数小于10％。

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