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'Hot' Macromolecular Crystals

机译：“热”高分子晶体

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摘要

Transcriptional regulator protein TM1030 from the hyperthermophile Thermotoga maritima, as well as its complex with DNA, was crystallized at it wide range of temperatures. Crystallization plates were incubated at 4, 20, 37, and 50 degrees C over 3 weeks. The best crystals of TM 1030 in complex with DNA were obtained at 4, 20, and 37 degrees C, while TM 1030 alone crystallized almost equally well in all temperatures. The crystals grown at different temperatures were used for X-ray diffraction experiments and their structures were compared. Surprisingly, the models of TM 1030 obtained from crystals grown at different temperatures are similar in quality. While there are some examples of structures of proteins grown at elevated temperatures in the PDB, these temperatures appear to be underrepresented. Our studies show that crystals of some proteins may be grown and are stable at broad range of temperatures. We suggest that crystallization experiments at elevated temperatures could be used as a standard part of the crystallization protocol.
机译：来自嗜热嗜热菌嗜热菌（Thermotoga maritima）的转录调节蛋白TM1030，以及它与DNA的复合物，在很宽的温度范围内都可以结晶。将结晶板在4、20、37和50℃下孵育3周。在10、4、20和37摄氏度下可获得与DNA复合的TM 1030的最佳晶体，而单独的TM 1030在所有温度下的结晶几乎相同。在不同温度下生长的晶体用于X射线衍射实验，并比较了它们的结构。令人惊讶的是，从在不同温度下生长的晶体获得的TM 1030的模型在质量上相似。虽然在PDB中有一些在高温下生长的蛋白质结构的例子，但这些温度似乎不足。我们的研究表明，某些蛋白质的晶体可能会生长，并在很宽的温度范围内保持稳定。我们建议可以将高温下的结晶实验用作结晶协议的标准部分。

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