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首页> 外文期刊>American Journal of Microbiology >Bioactive Endophytic Actinomycetes of Cinnamomum sp.; Isolation, Identification, Activity Guided Purification and Process Optimization of Active Metabolite
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Bioactive Endophytic Actinomycetes of Cinnamomum sp.; Isolation, Identification, Activity Guided Purification and Process Optimization of Active Metabolite

机译：生物活性内胚性放线菌肉桂瘤。 活性代谢物的分离，鉴定，活动引导纯化和过程优化

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摘要

Studying on endophytic actinomycetes of Cinnamomum sp. from Indian rainforest, Cherapunji, one of our isolated strains designated as CH1 was found to produce significant antibacterial activity against test pathogens (Aeromonas caviae (ATCC 15468), Vibrio parahemolyticus ATCC 1782, Pseudomonas aeruginosa (ATCC 9027), Proteus vulgaris (ATCC 12453), Shigella jlexnerii (ATCC 12022), Escherichia coli (human sample isolate), Bacillus subtilis (ATCC 11774) and Bacillus cereus (ATCC 14579). Scanning electron microscopic studies, morphological characterization and 16s ribosomal RNA gene sequence based classification strongly suggest that the isolate is a new strain of Streptomyces rochei. The rRNA gene sequenced was submitted to NCBI (GenBank accession number KJ486840.1). An antibacterial metabolite was extracted from fermented culture broth and purified by repeated silica gel column chromatography. TLC and bioautogram study of active fraction showed compound of Rf 0.196 was active metabolite where as HPLC study showed two major peak of RT 2.433 and 2.632 min. Parameters influencing optimal antimicrobial production were also determined and the active compound was found nonmutagenic by Ames test. Based on above experiments it is concluded that this entophytic isolate can be further exploited for industrial and biological applications.
机译：肉桂瘤的内生外形肌细胞学研究。从印度雨林，Cherapunji，我们的孤立菌株中的一个被认为是针对测试病原体的显着抗菌活性（Aeroomonas Caviae（ATCC 15468），Vibrio Parahemolyticus ATCC 1782，Pseudomonas Aeruginosa（ATCC 9027），ProteusVentgaris（ATCC 12453） ，Shigella jolexii（ATCC 12022），大肠杆菌（人样品分离物），枯草芽孢杆菌（ATCC 11774）和芽孢杆菌（ATCC 14579）。扫描电子显微镜研究，形态学表征和16S核糖体RNA基因序列的分类强烈表明隔离物是一种新的Streptomyces rochei菌株。rRNA基因测序被提交给NCBI（GenBank登录号KJ486040.1）。从发酵培养液中提取抗菌代谢物，并通过反复硅胶柱色谱法纯化。TLC和活跃部分的生物制止研究显示的RF 0.196的化合物是活性代谢物，其中HPLC研究显示出rt 2.433的两个主要峰d 2.632分钟。还测定了影响最佳抗微生物产量的参数，并通过AME试验发现活性化合物非培养。基于上述实验，得出结论，可以进一步利用这种营养性分离物用于工业和生物学应用。

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