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首页> 外文期刊>American Journal of Microbiology >Bioactive Endophytic Actinomycetes of Cinnamomum sp.; Isolation, Identification, Activity Guided Purification and Process Optimization of Active Metabolite
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Bioactive Endophytic Actinomycetes of Cinnamomum sp.; Isolation, Identification, Activity Guided Purification and Process Optimization of Active Metabolite

机译：Cinnamomum sp。的生物活性内生放线菌;活性代谢物的分离，鉴定，活性指导纯化和工艺优化

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摘要

Studying on endophytic actinomycetes of Cinnamomum sp. from Indian rainforest, Cherapunji, one of our isolated strains designated as CH1 was found to produce significant antibacterial activity against test pathogens (Aeromonas caviae (ATCC 15468), Vibrio parahemolyticus ATCC 1782, Pseudomonas aeruginosa (ATCC 9027), Proteus vulgaris (ATCC 12453), Shigella jlexnerii (ATCC 12022), Escherichia coli (human sample isolate), Bacillus subtilis (ATCC 11774) and Bacillus cereus (ATCC 14579). Scanning electron microscopic studies, morphological characterization and 16s ribosomal RNA gene sequence based classification strongly suggest that the isolate is a new strain of Streptomyces rochei. The rRNA gene sequenced was submitted to NCBI (GenBank accession number KJ486840.1). An antibacterial metabolite was extracted from fermented culture broth and purified by repeated silica gel column chromatography. TLC and bioautogram study of active fraction showed compound of Rf 0.196 was active metabolite where as HPLC study showed two major peak of RT 2.433 and 2.632 min. Parameters influencing optimal antimicrobial production were also determined and the active compound was found nonmutagenic by Ames test. Based on above experiments it is concluded that this entophytic isolate can be further exploited for industrial and biological applications.
机译：肉桂内生放线菌的研究。来自印度雨林Cherapunji，我们分离出的一种命名为CH1的菌株被发现对测试病原体（Aeromonas caviae（ATCC 15468），副溶血弧菌ATCC 1782，铜绿假单胞菌（ATCC 9027），寻常变形杆菌（ATCC 12453）产生了明显的抗菌活性，志贺氏菌（ATCC 12022），大肠杆菌（人类样品分离株），枯草芽孢杆菌（ATCC 11774）和蜡样芽孢杆菌（ATCC 14579）。扫描电子显微镜研究，形态学表征和基于16s核糖体RNA基因序列的分类强烈表明该分离株是罗氏链霉菌的新菌株，测序的rRNA基因已提交NCBI（GenBank登录号KJ486840.1），从发酵培养液中提取抗菌产物，并通过重复硅胶柱色谱法纯化，TLC和活性成分生物图谱研究表明Rf 0.196的化合物为活性代谢产物，其中HPLC研究显示RT 2.433的两个主要峰d 2.632分钟。还确定了影响最佳抗菌素生产的参数，并且通过Ames试验发现活性化合物无致突变性。基于上述实验，得出结论，该内生分离物可以进一步用于工业和生物学应用。

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