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首页> 外文期刊>Allergy >Casein-specific IL-4- and IL-13-secreting T cells: A tool to implement diagnosis of cow's milk allergy
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Casein-specific IL-4- and IL-13-secreting T cells: A tool to implement diagnosis of cow's milk allergy

机译：酪蛋白特异性IL-4-和IL-13分泌T细胞：一种实施牛奶过敏诊断的工具

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摘要

Results A total of 17 (59%) children reacted to cow's milk and were therefore considered as allergic to cow's milk (CMA). The mean number of casein-specific IL-4- and IL-13-secreting T cells was higher in CMA than in non-CMA children (P = 0.009, 0.004, respectively). Moreover, it was inversely correlated with the cumulative dose of cow's milk tolerated (P = 0.003, 0.0009, respectively). ROC curve of combined IL-4 and IL-13 analysis showed an area under the curve of 0.98 (95% CI 0.90-1.06). For a cutoff of 10 IL-4- and 12 IL-13-secreting T cells, sensitivity and negative predictive value were 100%.Conclusions Enumeration of casein-specific IL-4- and IL-13-secreting T cells appears a promising tool to improve diagnosis and, if confirmed in larger studies, could permit less frequent use of the oral food challenge.Background Cow's milk allergy (CMA) is a frequent food allergy in young children. The oral food challenge is the gold standard for diagnosis, and there is currently no reliable biological test. Our aim was to evaluate the diagnostic potential of a functional assay quantifying allergen-specific Th2 cells in CMA children.Methods A total of 29 children aged 2.8-10.5 years underwent a double-blind, placebo-controlled food challenge (DBPCFC) to cow's milk. Blood was collected before performing the DBPCFC, and peripheral mononuclear cells were cultured in an 18-h ELISpot assay with casein, α-lactalbumin, or β-lactoglobulin. Numbers of antigen-specific IL-4- and IL-13-secreting lymphocytes and serum-specific IgE, IgG4, and total IgE levels were assessed. Receiver operating characteristic (ROC) curves were generated.
机译：结果共17名（59％）儿童对牛奶反应，因此被视为对牛奶（CMA）过敏。 CMA的酪蛋白特异性IL-4-和IL-13分泌T细胞的平均数量高于非CMA儿童（P = 0.009,0.004）。此外，它与牛奶的累积剂量与耐受性的累积剂量相反（分别为0.003,0.0009）。联合IL-4和IL-13分析的ROC曲线显示了0.98曲线下的面积（95％CI 0.90-1.06）。对于10 IL-4和12 IL-13分泌T细胞的截止，敏感性和阴性预测值为100％。酪蛋白特异性IL-4-和IL-13分泌T细胞的枚举出现有望的工具为了改善诊断，如果在更大的研究中确认，可以允许更频繁地使用口腔食品挑战.Background Cow的牛奶过敏（CMA）是幼儿的常见食物过敏。口腔食品挑战是诊断的黄金标准，目前没有可靠的生物学测试。我们的目的是评估功能试验的诊断潜力量化CMA儿童中的过敏原特异性TH2细胞。均为2.8-10.5岁的29名儿童进行双盲，安慰剂对照食品挑战（DBPCFC）到牛奶。在进行DBPCFC之前收集血液，用酪蛋白，α-乳白蛋白酶或β-乳糖蛋白在18-H ELISPOT测定中培养外周单核细胞。评估抗原特异性IL-4-和IL-13分泌淋巴细胞和血清特异性IgE，IgG4和总IgE水平的数量。产生接收器操作特征（ROC）曲线。

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  • 来源
    《Allergy》 |2014年第11期|共8页
  • 作者

    MichaudB.; AroulandomJ.; BaizN.; AmatF.; Gouvis-EchraghiR.; CandonS.; ForayA.-P.; CoudercR.; BachJ.-F.; ChatenoudL.; JustJ.;

  • 作者单位

    Institut National de la Santé et de la Recherche MédicaleParis France Université Paris;

    Département d'Allergologie H?pital Trousseau AP-HP-UPMC Paris 6Paris France;

    Département d'Epidémiology des Maladies Allergiques et Respiratoires (EPAR) UMR-S 707 INSERM UPMC;

    Département d'Allergologie H?pital Trousseau AP-HP-UPMC Paris 6Paris France;

    Département d'Allergologie H?pital Trousseau AP-HP-UPMC Paris 6Paris France;

    Institut National de la Santé et de la Recherche MédicaleParis France Université Paris;

    Institut National de la Santé et de la Recherche MédicaleParis France Université Paris;

    Service de Biochimie et Biologie Moléculaire H?pital Trousseau AP-HPParis France;

    Institut National de la Santé et de la Recherche MédicaleParis France Université Paris;

    Institut National de la Santé et de la Recherche MédicaleParis France Université Paris;

    Département d'Allergologie H?pital Trousseau AP-HP-UPMC Paris 6Paris France Département d'Epid;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医学免疫学;
  • 关键词

    cow's milk allergy; IL-13; IL-4; Th2 cells;

    机译：牛奶过敏;IL-13;IL-4;TH2细胞;

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