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首页> 外文期刊>Allergy >Detailed characterization of Act d 12 and Act d 13 from kiwi seeds: Implication in IgE cross-reactivity with peanut and tree nuts
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Detailed characterization of Act d 12 and Act d 13 from kiwi seeds: Implication in IgE cross-reactivity with peanut and tree nuts

机译：来自猕猴桃的Act D 12和Act D 13的详细表征：用花生和树螺母的IgE交叉反应性含义

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Background Act d 12 (11S globulin) and Act d 13 (2S albumin) are two novel relevant allergens from kiwi seeds that might be useful to improve the diagnostic sensitivity and the management of kiwifruit-allergic patients.Objective To perform a comprehensive structural and immunological characterization of purified Act d 12 and Act d 13 from kiwi seeds.Methods Sera from 55 well-defined kiwifruit-allergic patients were used. Act d 12 and Act d 13 were purified by chromatographic procedures. Circular dichroism, mass spectrometry, concanavalin A detection, immunoblotting, enzyme-linked immunosorbent assays, basophil activation tests, and IgE-inhibition experiments were used.Results Act d 12 and Act d 13 were purified from kiwi seeds to homogeneity by combining size-exclusion, ion-exchange, and RP-HPLC chromatographies. Both purified allergens preserve the structural integrity and display typical features of their homologous counterparts from the 11S globulin and 2S albumin protein families, respectively. These allergens are released from kiwi seeds after oral and gastric digestion of whole kiwifruit, demonstrating their bioavailability after ingestion. The allergens retain the capacity to bind serum IgE from kiwifruit-allergic patients, induce IgE cross-linking in effector-circulating basophils, and display in vitro IgE cross-reactivity with homologous counterparts from peanut and tree nuts.Conclusion Purified Act d 12 and Act d 13 from kiwi seeds are well-defined molecules involved in in vitro IgE cross-reactivity with peanut and tree nuts. Their inclusion in component-resolved diagnosis of kiwifruit allergy might well contribute to improve the diagnostic sensitivity and the management of kiwifruit-allergic patients.
机译：背景动作D 12（11S球蛋白）和Act D 13（2S白蛋白）是来自猕猴桃种子的两种新的相关过敏原，这可能是改善诊断敏感性和猕猴桃过敏患者的诊断敏感性和管理。目的进行综合结构和免疫学纯化Act D 12的表征和来自猕猴桃的Act D 13.采用55种明确明确的猕猴桃过敏患者的血清。通过色谱法纯化Act D 12和Act D 13。使用圆形二色性，质谱，康吡甲酰胺检测，免疫印迹，酶联免疫吸附试验，嗜碱性激活试验和IgE抑制实验。通过组合尺寸排除，从猕猴桃种子纯化了作用D 12和Act D 13 ，离子交换和RP-HPLC色谱分类。纯化过敏原均采用结构完整性，分别从11S球蛋白和2S白蛋白蛋白质家族的同源对应物的典型特征。这些过敏原在口服和胃消化后从猕猴桃种子释放，在摄入后展示了它们的生物利用度。过敏原保留从猕猴桃 - 过敏患者结合血清IgE的能力，诱导Ige循环嗜碱性粒细胞中的IgE交联，并用来自花生和树坚果的同源对应物显示体外IgE交叉反应性。结论纯化的动作D 12和Act来自猕猴桃的D 13是与花生和树螺母的体外IgE交叉反应性涉及明确定义的分子。它们纳入组分解决的猕猴桃过敏的诊断可能会有助于提高诊断敏感性和Kiwifruit-过敏患者的管理。

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