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首页> 外文期刊>Animal Science Journal >Microarray analysis of Longissimus thoracis muscle gene expressions in vitamin A-restricted Japanese Black steers in middle fattening stage
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Microarray analysis of Longissimus thoracis muscle gene expressions in vitamin A-restricted Japanese Black steers in middle fattening stage

机译：中育阶段维生素A限制日本黑色阉牛骨肌肉基因表达的微阵列分析

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摘要

Vitamin A (VA) restriction in beef cattle improves meat marbling; however, the underlying molecular mechanisms remain incompletely understood. We performed microarray analysis to clarify the effect of VA restriction on Longissimus thoracis gene expressions in Japanese Black steers. Six Japanese Black steers 13-14 months of age were divided into two groups: S group (n = 3), which received VA supplementation, and R group ( n = 3), in which dietary VA intake was restricted. Steers were fattened for 7 months, following which tissue samples were obtained. Extracted RNA samples were analyzed by Affymetrix Genechip Bovine Genome Array. Lists of genes highly expressed in the R and S groups were obtained. The lists were functionally interpreted using functional annotation software, DAVID. In the R and S groups, 48 and 40 genes were significantly highly expressed, respectively. The gene list of the R group included CD36, LPL, GPAM, DGAT2, and SCD and additional genes annotated 'PPAR signaling pathway,' 'lipid biosynthesis' and 'mitochondrion,' whereas that of the S group included COL1A2, FN1 and DCN and additional genes annotated ' extracellular matrix.' Changes in the expression of these genes are possibly involved in marbling improvement in beef cattle by VA restriction.
机译：维生素A（VA）在牛肉中的限制改善了肉织大理石;然而，潜在的分子机制仍然不完全理解。我们进行了微阵列分析，以阐明VA限制对日本黑色阉牛体的Longissimus Thoracis基因表达的影响。 13-14个月年龄的六个日本黑色阉牛分为两组：S组（n = 3），该组（n = 3），其收到VA补充，r组（n = 3），其中膳食VA Intake受到限制。阉叶浓缩7个月，然后获得组织样品。通过Affymetrix GeneChip牛基因组阵列分析提取的RNA样品。获得了R和S组高度表达的基因列表。列表使用功能注释软件David功能解释。在R和S组中，分别显着表达48和40个基因。 R组的基因列表包括CD36，LPL，GPAM，DGAT2和SCD和另外的基因注释了'PPAR信号通路，'脂生物合成'和'线粒体'，而S组包括COL1A2，FN1和DCN和另外的基因注释了“细胞外基质”。这些基因表达的变化可能参与VA限制牛肉牛的大理石改善。

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