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首页> 外文期刊>Analytical Letters >Determination of Chlorogenic Acid and Rutinum in Herba Artemisiae Scopariae with Multitemplate Molecularly Imprinted Polymers for Solid-phase Extraction with High-performance Liquid Chromatography
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Determination of Chlorogenic Acid and Rutinum in Herba Artemisiae Scopariae with Multitemplate Molecularly Imprinted Polymers for Solid-phase Extraction with High-performance Liquid Chromatography

机译：用高效液相色谱法测定Herba Artemisiae Scopariae中的绿原酸和芦丁瘤的含量，高效液相色谱法

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摘要

Novel multitemplate molecularly imprinted polymers were prepared using mixtures of chlorogenic acid and rutinum as molecular templates, acrylamide as a functional monomer, divinylbenzene as the cross-linker, and 20:80 methanol:acetone as the porogen. The polymers were assessed for solid-phase extraction (SPE) for the purification of two compounds from Herba Artemisiae Scopariae. The synthesized molecularly imprinted polymers were identified by infrared spectroscopy and scanning electron microscopy. Systematic characterization of the functional monomer and porogens on the recognition properties of the molecularly imprinted polymers were carried out. Comparison with single-template molecularly imprinted polymers showed that the multitemplate molecular polymers exhibited higher selectivity and adsorption capacity for multiple analytes. The optimization of washing solvent as 1:9 acetone:water and the elution solvent as 9:1 acetonitrile:acetic acid provided a reliable analytical method with strong recognition toward multiple analytes in Herba Artemisiae Scopariae extracts with satisfactory recoveries of 89.6% for chlorogenic acid and 93.8% for rutinum. These results demonstrate that the multitemplate molecularly imprinted polymers coupled with SPE are effective for the selective purification of bioactive compounds in complex samples.
机译：使用绿原酸和芦丁的混合物作为分子模板，丙烯酰胺作为官能单体，二乙烯基苯作为交联剂，20:80作为致孔剂的丙酮，制备新的多层膜的分子印迹聚合物。评估聚合物用于固相萃取（SPE），用于纯化来自Herba Artemisiae Scopariae的两种化合物。通过红外光谱和扫描电子显微镜鉴定合成的分子印迹聚合物。进行了在分子印迹聚合物的识别性质上的功能性单体和散孔的系统特征。与单模分子印迹聚合物的比较表明，多层膜子聚合物表现出多种分析物的选择性和吸附能力较高。优化洗涤溶剂为1：9丙酮：水和洗脱溶剂，为9：1乙腈：乙酸提供了一种可靠的分析方法，具有强烈识别Herba Artemisiae Scopariae提取物的多个分析物，其令人满意的复位为绿原酸89.6％乌丁南的93.8％。这些结果表明，与SPE偶联的多层膜的分子印迹聚合物是有效的，用于在复杂样品中选择性纯化生物活性化合物。

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