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首页> 外文期刊>Analytical Biochemistry: An International Journal of Analytical and Preparative Methods >Principal component analysis of MALDI-TOF MS of whole-cell foodborne pathogenic bacteria
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Principal component analysis of MALDI-TOF MS of whole-cell foodborne pathogenic bacteria

机译：全细胞食源性致病菌MALDI-TOF MS的主要成分分析

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摘要

The rapid and accurate identification of foodborne pathogenic bacteria is of great importance for human health. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) can be used to rapidly and sensitively identify microorganisms but is limited by the expensive protein databases available. In this study, we established a whole-cell method for the identification of foodborne pathogenic bacteria, using MALDI-TOF MS and principal component analysis (PCA), which did not use protein extractions or expensive protein databases. Thirty strains comprising six common foodborne pathogenic bacteria, namely, Shigella flexneri, Escherichia coll., Staphylococcus aureus, Salmonella enteritidis, Pseudomonas aeruginosa, and Listeria monocytogenes were analyzed using MALDI-TOF MS. The culture time, matrix, and spotting method were optimized based on peak intensity and deviation. A PCA was performed to analyze the mass spectrometry results of six samples and proved capable of identifying significant changes in those samples. It was found that directly applying MALDI-TOF MS analysis to whole-cell bacteria, without protein extraction, exhibited rich peak contents and a high level of reproducibility. MALDI-TOF MS combined with PCA is a promising method of rapidly identifying pathogens in food products.
机译：快速准确地鉴定食品致病菌性致病菌对人类健康具有重要意义。基质辅助激光解吸/电离 - 飞行量谱（MALDI-TOF MS）可用于快速敏感地鉴定微生物，但受到昂贵的蛋白质数据库的限制。在这项研究中，我们建立了一种使用MALDI-TOF MS和主要成分分析（PCA）来鉴定食品致病菌的全细胞方法，该方法不使用蛋白质提取或昂贵的蛋白质数据库。使用MALDI-TOF MS分析，包括六颗普通植物，即Shigella Flexeri，大肠杆菌，沙门氏菌，静脉铜绿假单胞菌和Histeria单核细胞生成的血糖菌。基于峰强度和偏差优化培养时间，矩阵和斑点方法。进行PCA以分析六个样品的质谱结果，并证明能够识别这些样品的显着变化。发现，没有蛋白质萃取的全细胞细菌直接将MALDI-TOF MS分析应用于富蛋白质萃取，并且具有丰富的峰含量和高水平的重现性。 MALDI-TOF MS与PCA相结合是一种快速识别食品中病原体的有希望的方法。

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