...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Andrologia >Impact of alcohol and cigarette smoking consumption in male fertility potential: Looks at lipid peroxidation, enzymatic antioxidant activities and sperm DNA DNA damage
【24h】

Impact of alcohol and cigarette smoking consumption in male fertility potential: Looks at lipid peroxidation, enzymatic antioxidant activities and sperm DNA DNA damage

机译：酒精和香烟吸烟在男性生育潜力中的影响：看脂质过氧化，酶促抗氧化活性和精子DNA DNA损伤

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Summary Alcohol intake and cigarette smoking are the major lifestyle factors with negative impact on fertility. We were interested to evaluate the negative impact of these factors on oxidative stress ( OS ), enzymatic antioxidant activity ( EAO ) of spermatozoa and on its DNA damage. This study included 108 male infertile patients with normal range of sperm conventional parameters but with unexplained infertility in assisted reproductive technologies programme. Firstly, OS was analysed based on lipid peroxidation ( MDA ) and EAO which included catalase ( CAT ), superoxide dismutase ( SOD ) and glutathione reductase ( GR ). Secondly, we evaluated DNA fragmentation by TUNEL assay and chromatin decondensation by aniline blue colouration. The whole lot was divided into four groups: control (nonalcoholic and nonsmoker patients), alcohol group, smoking group and alcohol‐smoking group. The results showed, in three last groups compared to control an increased CAT , SOD and GR activities with high MDA level especially in smoking and alcohol‐smoking group. The latter showed the highest values of DNA fragmentation and chromatin decondensation (31% and 39%) to exceed DNA damage normal range. Indeed, smoking and alcohol intake lead to increase EAO due to long‐term unbalanced antioxidant/oxidation ratio with high OS which cause consequently sperm DNA damage calling in need by urgency to change the lifestyle behaviour.
机译：摘要酒精摄入和吸烟是对生育的主要生活方式因素。我们有兴趣评估这些因素对氧化应激（OS），精子酶抗氧化活性（EAO）和其DNA损伤的负面影响。本研究包括108名男性不孕症患者，具有正常的精子常规参数，但在辅助生殖技术计划中具有无法解释的不孕症。首先，基于脂质过氧化（MDA）和eao分析OS，其包括过氧化氢酶（猫），超氧化物歧化酶（SOD）和谷胱甘肽还原酶（GR）。其次，我们通过苯胺蓝色聚色评估Tunel测定和染色质裂解的DNA碎片。整个批次分为四组：控制（非酒精和非酒歌患者），酒精组，吸烟组和吸烟组。结果显示，在最后一组群体中，与对照具有高MDA水平的增加的猫，草皮和GR活动，特别是在吸烟和吸烟组中。后者显示DNA碎片和染色质裂解（31％和39％）的最高值以超过正常范围的DNA损伤。实际上，由于长期不平衡抗氧化剂/氧化比率，吸烟和酒精摄入量导致EAO增加，这导致精子DNA损伤所需的呼吁改变生活方式行为。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号