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Sortase A: A Model for Transpeptidation and Its Biological Applications

机译:排序序列A:跨缺陷和生物应用模型

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Molecular biologists and chemists alike have long sought to modify proteins with substituents that cannot be installed by standard or even advanced genetic approaches. We here describe the use of transpeptidases to achieve these goals. Living systems encode a variety of transpeptidases and peptide ligases that allow for the enzyme-catalyzed formation of peptide bonds, and protein engineers have used directed evolution to enhance these enzymes for biological applications. We focus primarily on the transpeptidase sortase A, which has become popular over the past few years for its ability to perform a remarkably wide variety of protein modifications, both in vitro and in living cells.
机译:分子生物学家和化学家长期以来,我们希望改性蛋白质与不能通标准甚至先进的遗传方法安装的取代基。 我们在这里描述了使用传递速度酶来实现这些目标。 活体系编码各种透肽酶和肽连接酶,其允许酶催化的肽键的形成,蛋白质工程师使用定向的进化来增强这些酶的生物应用。 我们主要关注在过去几年中的转琥珀酶分子A A,其能够在体外和活细胞中进行显着各种蛋白质修饰的能力。

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