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首页> 外文期刊>Acta Physiologiae Plantarum >Molecular cloning and expression analysis of two FAD2 genes from chia (Salvia hispanica)
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Molecular cloning and expression analysis of two FAD2 genes from chia (Salvia hispanica)

机译：两种FAD2基因的分子克隆及表达分析来自Chia（Salvia Hispanica）

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摘要

FATTY ACID DESATURASE 2 (FAD2, EC 1.3.1.35), also known as delta-12 oleate desaturase, is a key enzyme for linoleic acid and alpha-linolenic acid biosynthesis. Chia (Salvia hispanica) seeds contain the highest known proportion of a-linolenic acid in any plant sources. In this study, two full-length FAD2 genes, named as ShFAD2-1 and ShFAD2-2, were isolated from S. hispanica based on RACE method. Both ShFAD2-1 and ShFAD2-2 proteins possess strong transmembrane helices, three histidine motifs and a C-terminal ER-located signal (YNNKL). Phylogenetic analysis showed that both ShFAD2-1 and ShFAD2-2 are grouped with constitutive plant FAD2s. Heterologous expression in Saccharomyces cerevisiae indicated that ShFAD2-1 and ShFAD2-2 genes both encode a bio-functional delta-12 oleate desaturase. ShFAD2-2 was mainly expressed in flowers and early-stage seeds while ShFAD2-1 expression was almost constitutive in different organs. qRT-PCR results demonstrated that ShFAD2-1 and ShFAD2-2 show a cold-induced and heat-repressed expression pattern, whereas they also were differentially up-regulated or repressed by other abiotic stresses. This is the first cloning and function characterization of FAD2 from S. hispanica, which can provide insights into molecular mechanism of high ALA traits of S. hispanica and enrich our understanding of the roles of FAD2 genes in various abiotic stresses.
机译：脂肪酸去饱和酶2（FAD2，EC 1.3.1.35），也称为DELTA-12糖酸酯酶，是亚油酸和α-亚麻酸生物合成的关键酶。 Chia（Salvia Hispanica）种子在任何植物源中含有最高已知的α-亚麻酸比例。在本研究中，基于种族方法从S. Hispanica中分离出两个名为Shfad2-1和Shfad2-2的两个全长FAD2基因。 SHFAD2-1和SHFAD2-2蛋白质均具有强大的跨膜螺旋，三个组氨酸基序和C末端ER定位信号（YNNK1）。系统发育分析表明，SHFAD2-1和SHFAD2-2均用组成型植物FAD2S分组。酿酒酵母中的异源表达表明，SHFAD2-1和SHFAD2-2基因两者都编码生物功能δ-12含油酶去饱和酶。 SHFAD2-2主要用鲜花和早期种子表达，而SHFAD2-1表达在不同器官中几乎是本质载体。 QRT-PCR结果表明，SHFAD2-1和SHFAD2-2显示出冷诱导和热抑制的表达模式，而它们也被其他非生物应激差异上调或压制。这是来自S. Hispanica的FAD2的第一个克隆和功能表征，其可以提供对Heapanica的高ALA性状的分子机制的见解，并丰富我们对FAD2基因在各种非生物胁迫中的作用的理解。

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