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首页> 外文期刊>Acta veterinaria. >BLOCKADE OF P-SELECTIN REDUCES NEUTROPHIL INFILTRATION INTO THE ISCHEMIA-REPERFUSION INDUCED MURINE TESTIS
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BLOCKADE OF P-SELECTIN REDUCES NEUTROPHIL INFILTRATION INTO THE ISCHEMIA-REPERFUSION INDUCED MURINE TESTIS

机译：P-选择素的阻滞减少中性粒细胞浸润进入缺血再灌注诱导的小鼠睾丸

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摘要

Germ cell apoptosis after ischemia-reperfusion (IR) of the testis is dependent on neutrophil recruitment to the testis. Intravascular adhesion molecules like the P- and E- selectins play an important role in this recruitment. The purpose of this study was to inhibit neutrophil recruitment to the IR- induced testis by using a function-blocking monoclonal anti-mouse P-selectin antibody.Adult mice were subjected to a 2 h period of testicular torsion (ischemia) followed by detorsion (reperfusion). Ten minutes after the onset of reperfusion mice received either the function-blocking monoclonal P-selectin antibody (FBMAB group) or the isotype-matched control antibody (IMCA group). Separate groups of mice underwent a sham operation (SO group) or received 500 ng of TNF alpha (IF group) to induce inflammation.Mice were sacrificed 24 hr after reperfusion and testiscular interstitial cells were isolated and analyzed for the presence of neutrophils by means of flow cytometry.The function-blocking monoclonal P-selectin antibody reduced neutrophil recruitment to the IR-induced testis significantly. Therefore, blocking P-selectin may be therapeutically beneficial to protect postischemic testis.
机译：睾丸缺血再灌注（IR）后生殖细胞凋亡取决于嗜中性粒细胞募集到睾丸。血管内黏附分子，例如P-和E-选择素在这种募集中起重要作用。这项研究的目的是通过使用功能阻断性单克隆抗小鼠P-选择素抗体来抑制嗜中性白细胞募集到IR诱导的睾丸中。成年小鼠经历2小时的睾丸扭转（局部缺血），然后扭曲（再灌注）。在再灌注发作后十分钟，小鼠接受了功能阻断性单克隆P-选择素抗体（FBMAB组）或同型匹配的对照抗体（IMCA组）。分别进行假手术组（SO组）或接受500 ng TNFα（IF组）诱导炎症反应。再灌注后24小时处死小鼠，分离睾丸间质细胞，并通过以下方法分析中性粒细胞的存在阻断功能的单克隆P-选择素抗体可显着减少中性粒细胞向IR诱导的睾丸的募集。因此，阻断P-选择蛋白在治疗缺血性睾丸方面可能有益。

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