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首页> 外文期刊>DTW:Deutsche Tierarztliche Wochenschrift >Blockade of p-selectin reduces neutrophil infiltration into the murine testis after ischemia-reperfusion-injury
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Blockade of p-selectin reduces neutrophil infiltration into the murine testis after ischemia-reperfusion-injury

机译：对p-选择素的阻断可减少缺血再灌注损伤后中性粒细胞向鼠睾丸的浸润

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摘要

Germ cell specific apoptosis after ischemia-reperfusion (I/R) induced testicular injury is dependent on neutrophil recruitment to the testis. Intravascular adhesion molecules like the P- and E-selectins play an important role in this recruitment. The purpose of this study was to inhibit neutrophil recruitment in I/R induced testicular injury by using a function-blocking monoclonal anti-mouse P-selectin antibody, Adult mice were subjected to a 2 h period of testicular torsion (ischemia) followed by detorsion (reperfusion). Ten minutes after the onset of reperfusion, mice received either 100 mu g of a function-blocking monoclonal P-selectin antibody (FBMAB group) or isotype-matched control antibody (IMCA group). Separate groups of mice underwent sham-operation (SO group) or received 500 ng of TNF alpha (IF group) to induce inflammation, Mice were sacrificed 24 h after reperfusion and testiscular interstitial cells were isolated and analyzed for the presence of neutrophils by means of flow cytometry.The function-blocking monoclonal P-selectin antibody reduced neutrophil recruitment in A induced testicular injury significantly (FBMAB group as compared to the IMCA group 26 +/- 4 vs. 52 +/- 10% Gr-1 + CD11b+ of total leucocytes; P < 0.001),Therefore, blocking P-selectin may be therapeutically beneficial to protect postischemic testis.
机译：缺血再灌注（I / R）引起的睾丸损伤后生殖细胞特异性凋亡取决于中性粒细胞募集到睾丸。血管内黏附分子（如P-选择素和E-选择素）在这种募集中起重要作用。这项研究的目的是通过使用功能阻断性单克隆抗小鼠P-选择素抗体来抑制I / R诱导的睾丸损伤中的中性粒细胞募集。成年小鼠经历2 h的睾丸扭转（缺血），然后发生扭曲（再灌注）。再灌注开始后十分钟，小鼠接受100μg功能阻断性单克隆P-选择素抗体（FBMAB组）或同种型匹配的对照抗体（IMCA组）。分别对小鼠进行假手术（SO组）或接受500 ng TNFα（IF组）诱导炎症，在再灌注后24 h处死小鼠，分离睾丸间质细胞，并通过以下方法分析中性粒细胞的存在阻断功能的单克隆P-选择素抗体可显着降低A诱导的睾丸损伤中的中性粒细胞募集（FBMAB组比IMCA组26 +/- 4 vs.52 +/- 10％Gr-1 + CD11b +白细胞; P <0.001），因此，阻断P-选择素可能在保护缺血性睾丸方面具有治疗益处。

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