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首页> 外文期刊>Applied Biological Chemistry >Schisandra chinensis inhibiting TGF-induced activation of hepatic stellate cells
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Schisandra chinensis inhibiting TGF-induced activation of hepatic stellate cells

机译：Schisandra Chinensis抑制TGF诱导的肝星状细胞的活化

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摘要

Hepatic fibrosis is one of the critical steps contained in the pathogenesis of liver cirrhosis. Excessive deposition of collagen contributes to the development of fibrosis in chronic liver injury. Activation of hepatic stellate cells (HSCs) plays an important role in fibrogenesis and is accountable for providing extracellular matrix components. The berry of Schisandra chinensis has been known to exert hepatoprotective properties. However, its effect on HSCs is not completely understood. Therefore, in this study, we investigated the inhibitory effect of its ethanolic extract (SBE) on hepatic fibrogenesis. We found that SBE treatment effectively reduced the serum levels of alanine aminotransferase and aspartate aminotransferase as well as collagen deposition in the hepatic parenchyma in a thioacetamide-induced hepatic fibrosis mouse model. Moreover, SBE inhibited transforming growth factor (TGF)-induced mRNA expression of -smooth muscle actin (SMA) and collagen type 1 1 (COL1A1) in HSCs, suggesting that SBE exerts anti-fibrotic activity by attenuating TGF-induced HSC activation. To identify the active components of SBE accountable for HSC inhibition, SBE was further partitioned based on the hydrophobicity of the solvents such as water, n-butanol, ethyl acetate, chloroform, and n-hexane. The n-hexane fraction was selected and further separated using analytical high-performance liquid chromatography. We found that six lignans contained in the n-hexane fraction strongly reduced TGF-induced expression of both SMA and COL1A1 mRNA. These data suggest that at least six lignans contained in SBE have the strong potential to prevent TGF-induced HSC activation.
机译：肝纤维化是肝硬化发病机制中所含的关键步骤之一。胶原蛋白的过度沉积有助于慢性肝损伤纤维化的发展。肝星状细胞（HSCs）的活化在纤维发生中起重要作用，并且是为提供细胞外基质组分的负责任。已知Schisandra Chinensis的浆果施用肝脏保护性。然而，它对HSCs的影响并不完全理解。因此，在本研究中，我们研究了乙醇提取物（SBE）对肝纤维发生的抑制作用。我们发现SBE治疗有效地降低了丙酸酰胺诱导的肝脏纤维化小鼠模型中肝实质中的丙氨酸氨基转移酶和天冬氨酸氨基转移酶的血清水平。此外，SBE抑制转化生长因子（TGF） - 在HSC中抑制了-Smooth肌肉肌动蛋白（SMA）和胶原蛋白1 1（COL1A1）的mRNA表达，表明SBE通过衰减TGF诱导的HSC活化来施加抗纤维化活性。为了鉴定HSC抑制的SBE的活性组分，基于溶剂如水，正丁醇，乙酸乙酯，氯仿和正己烷等溶剂进一步分配SBE。选择N-己烷级分，并使用分析高效液相色谱法进一步分离。我们发现N-己烷级分中含有六个木质素，强烈降低了SMA和COL1A1 mRNA的TGF诱导的表达。这些数据表明，SBE中至少有六个含有的木质素具有强烈的潜力来预防TGF诱导的HSC活化。

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