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首页> 外文期刊>Antonie van Leeuwenhoek: Journal of Microbiology and serology >Comparative transcriptomic analysis identified differentially expressed genes and pathways involved in the interaction between Tremella fuciformis and Annulohypoxylon stygium
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Comparative transcriptomic analysis identified differentially expressed genes and pathways involved in the interaction between Tremella fuciformis and Annulohypoxylon stygium

机译：比较转录组分析鉴定了差异表达的基因和涉及Interla Fucifordis和Annulohypoxylon Stegium相互作用的途径

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摘要

Tremella fuciformis is an edible and medicinal white jelly mushroom. It has a life cycle that interacts with its companion fungus Annulohypoxylon stygium, both in natural conditions and artificial cultivation. RNA sequencing (RNA-Seq) was used to study the interaction between T. fuciformis and A. stygium by constructing 5 libraries, including the individual T. fuciformis mycelium (1), the T. fuciformis mycelium after interaction with A. stygium (2), the dual mycelia after interaction (3), the A. stygium mycelium after interaction with T. fuciformis (4), and the individual A. stygium mycelium (5). 33.4 G data and 46,871 Unigenes were generated from de novo splicing. For identification of differentially expressed genes (DEGs) related to interaction, we analyzed the expression data of DEGs1-vs-2 boolean AND DEGs1-vs-3, and DEGs5-vs-4 boolean AND DEGs5-vs-3. DEGs1-vs-2 boolean AND DEGs1-vs-3, and DEGs5-vs-4 boolean AND DEGs5-vs-3 data showed 614 DEGs and 1537 DEGs, respectively. The 614 DEGs for T. fuciformis and 1537 DEGs for A. stygium were analyzed by GO annotation and were assigned to biology process, cell composition, and molecular functions. The DEGs were used to match the KEGG database. In T. fuciformis, the pathways are primarily enriched various amino acids metabolism, pentose and glucuronate interconversions. In A. stygium, the pathways are primarily enriched in the biosynthesis of secondary metabolites, biosynthesis of antibiotics, starch and sucrose metabolism. The expression patterns of DEGs determined by qRT-PCR were consistent with those obtained by RNA-Seq, thus validating the reliability of our RNA-Seq data. Future studies of the functions of these interesting genes will be helpful to understand the mechanisms by which T. fuciformis interacts with A. stygium. This will also provide a reference for other research on interacting microorganisms.
机译：金蝶菌是一种可食用和药用白果冻蘑菇。它具有生命周期，其与其同伴的真菌annulohypoxylys散，既是天然条件和人工培养。使用RNA测序（RNA-SEQ）研究T.Fucifordis和A.Stygium之间的相互作用，通过构建5种文库，包括单独的T.Fucifordis菌丝体（1），T.Fuciformis菌丝体与A. Stegium相互作用（2 ），双菌丝体相互作用（3），A.与T. fucifordis（4）的相互作用后的菌丝体和单独的菌丝体（5）。从De Novo剪接产生33.4g数据和46,871个unigenes。为了鉴定与交互相关的差异表达基因（DEGS），我们分析了DEGS1-VS-2布尔和DEGS1-VS-3的表达数据，以及DEGS5-VS-4布尔和DEGS5-VS-3。 DEGS1-VS-2布尔和DEGS1-VS-3，以及DEGS5-VS-4布尔和DEGS5-VS-3数据分别显示614°和1537次。通过GO注释分析了614°F for T. Fucifordis和1537℃的嗜锑，并分配给生物学过程，细胞组合物和分子函数。 DEGS用于匹配KEGG数据库。在T.Fuciformis中，途径主要富集各种氨基酸代谢，戊糖和葡糖醛酸酯互联。在A. Stegium中，途径主要是富集的次级代谢物的生物合成，抗生素生物合成，淀粉和蔗糖代谢。由QRT-PCR确定的DEG的表达模式与RNA-SEQ获得的那些一致，从而验证我们的RNA-SEQ数据的可靠性。未来对这些有趣基因的功能的研究将有助于了解T. Fucifordis与A. Stegium互动的机制。这还将提供关于相互作用微生物的其他研究的参考。

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