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首页> 外文期刊>Aquaculture Research >Estimation of genetic variation in green tiger prawn, Penaeus semisulcatus by using random amplified polymorphic DNA, inter simple sequence repeat and simple sequence repeat markers
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Estimation of genetic variation in green tiger prawn, Penaeus semisulcatus by using random amplified polymorphic DNA, inter simple sequence repeat and simple sequence repeat markers

机译：用随机扩增多态性DNA估计绿虎虾，彭诺·苏拉肌的遗传变异，简单序列重复和简单序列重复标记

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摘要

In this study, three molecular markers including random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR) and simple sequence repeat (SSR) were used to evaluate genetic variation of green tiger prawn Penaeus semisulcatus collected from two geographically isolated environments; located in the Manifa, Arabian Gulf, Saudi Arabia and Ataka, Suez Gulf, Egypt. Genetic parameters included the percentage of polymorphism (P%), effective alleles (Ne), Nei genetic diversity (H) and Shannon index (I), which were calculated based on molecular data. All three marker systems distinguished genetic variation of P. semisulcatus in various levels. The highest polymorphism (91.30%) was obtained with SSR, followed by ISSR (82.26%) and RAPD markers (62.04%), respectively. Our results indicate that SSR appeared to be the best suited molecular assay for assessing the genetic variation between genotypes of P. semisulcatus. The present study indicated that Manifa and Ataka genotypes were closely related. Moreover, the analysis of variability could require more than one DNA-based molecular marker techniques.
机译：在该研究中，使用包括随机扩增多晶态DNA（RAPD）的三种分子标记，简单的序列重复（ISSR）和简单的序列重复（SSR）评估从两个地理上孤立的环境中收集的绿色虎虾Penaeus Semisulcatus的遗传变异;位于数数，阿拉伯海湾，沙特阿拉伯和Aataka，苏伊斯湾，埃及。遗传参数包括基于分子数据计算的多态性（P％），有效等位基因（NE），NEI遗传多样性（H）和Shannon指数（I）的百分比。所有三种标记系统在各种水平中分别遗传变异。用SSR获得最高的多态性（91.30％），其次是ISSR（82.26％）和RAPD标记（62.04％）。我们的结果表明，SSR似乎是评估P. Semisulcatus基因型之间的遗传变异的最适合的分子测定。本研究表明，司目和ATAKA基因型密切相关。此外，可变性的分析可能需要多于一种基于DNA的分子标记技术。

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