Direct current electrical stimulation chamber for treating cells in vitro

Mobini Sahba; Leppik Liudmila; Barker John H.

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Direct current electrical stimulation chamber for treating cells in vitro

机译：直流电刺激室，用于体外处理细胞

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Electrical stimulation has been shown to promote healing and regeneration in skin, bone, muscle, and nerve tissues in clinical studies. Recently, studies applying electrical stimulation to influence cell behavior associated with proliferation, differentiation, and migration have provided a better understanding of the underlying mechanisms of electrical stimulation-based clinical treatments and improved tissue-engineered products through electro-bioreactor technologies. Here, we present a novel device for delivering direct current (DC) electrical stimulation (ES) to cultivated cells in vitro. Our simplified electro-bioreactor is customized for applying DC electrical current simultaneously in six individual tissue culture wells. The design overcomes previous experimental replicate limitations, thus reducing experimental time and cost.

机译：在临床研究中，电刺激已显示可促进皮肤，骨骼，肌肉和神经组织的愈合和再生。最近，应用电刺激影响与增殖，分化和迁移相关的细胞行为的研究，通过电生物反应器技术，对基于电刺激的临床治疗和改进的组织工程产品的潜在机制有了更好的了解。在这里，我们提出了一种新颖的装置，可将直流电（DC）电刺激（ES）传递到体外培养的细胞。我们简化的电子生物反应器经过定制，可在六个单独的组织培养孔中同时施加直流电流。该设计克服了以前的实验复制限制，从而减少了实验时间和成本。

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来源
《BioTechniques》 |2016年第2期|共4页
作者
Mobini Sahba; Leppik Liudmila; Barker John H.;
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正文语种 eng
中图分类分子生物学;
关键词
DC electrical stimulation; in vitro; tissue engineering; bioreactors; osteogenic differentiation;

机译：直流电刺激;体外;组织工程;生物反应器;成骨分化;

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专利

1. Direct current electrical stimulation chamber for treating cells in vitro [J] . Mobini Sahba, Leppik Liudmila, Barker John H. BioTechniques . 2016,第2期

机译：直流电刺激室，用于体外处理细胞
2. Does Transcranial Direct Current Stimulation Combined with Peripheral Electrical Stimulation Have an Additive Effect in the Control of Hip Joint Osteonecrosis Pain Associated with Sickle Cell Disease? A Protocol for a One-Session Double Blind, Block-Randomized Clinical Trial [J] . Tiago da Silva Lopes, Wellington dos Santos Silva, Sanzia B. Ribeiro, Frontiers in Human Neuroscience . 2017,第12期

机译：经颅直流电刺激与周围电刺激相结合是否对控制镰状细胞病相关的髋关节骨坏死疼痛具有相加作用？一站式双盲，随机分组临床试验的方案
3. Noninvasive Brain Stimulation with Low-Intensity Electrical Currents: Putative Mechanisms of Action for Direct and Alternating Current Stimulation [J] . Soroush Zaghi Mariana Acar Brittney Hultgren Paulo S. Boggio and Felipe Fregni The Neuroscientist: a review journal bringing neurobiology, neurology and psychiatry . 2010,第3期

机译：低强度电流的非侵入性脑刺激：直接和交流刺激的推定作用机制
4. Alignment and Elongation of Human Adipose-Derived Stem Cells in Response to Direct-Current Electrical Stimulation [C] . Nina Tandon, Brian Goh, Anna Marsano, Annual International Conference of the IEEE Engineering in Medicine and Biology Society . 2009

机译：响应于直流电刺激的人脂肪衍生的干细胞的对准和伸长
5. Effects of alternating current electrical stimulation on the cellular chemistry and proliferation of C2C12 muscle cells [D] . Koh, Kar Mun. 2016

机译：交流电刺激对C2C12肌肉细胞化学和增殖的影响
6. In vitro effect of direct current electrical stimulation on rat mesenchymal stem cells [O] . Sahba Mobini, Liudmila Leppik, Vishnu Thottakkattumana Parameswaran, -1

机译：直流电刺激对大鼠间充质干细胞的体外作用
7. In vitro effect of direct current electrical stimulation on rat mesenchymal stem cells [O] . Sahba Mobini, Liudmila Leppik, Vishnu Thottakkattumana Parameswaran, 2017

机译：直流电刺激对大鼠骨髓间充质干细胞的体外作用
8. Electrical Stimulation Enhances Cellular/Molecular Functions of Osteoblasts Relevant to New Bone Formation in Vitro. [R] . Supronowicz, P. R., Ullmann, K. R., Ajayan, P. M., 2001

机译：电刺激增强成骨细胞的细胞/分子功能与体外新骨形成有关。

Direct current electrical stimulation chamber for treating cells in vitro

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