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首页> 外文期刊>Applied Soil Ecology >Next generation bioformulation prepared by amalgamating Bradyrhizobium, cell free culture supernatant, and exopolysaccharides enhances the indigenous rhizospheric rhizobial population, nodulation, and productivity of pigeon pea
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Next generation bioformulation prepared by amalgamating Bradyrhizobium, cell free culture supernatant, and exopolysaccharides enhances the indigenous rhizospheric rhizobial population, nodulation, and productivity of pigeon pea

机译：下一代通过合并浆化的Bradyrhizobium，无细胞培养上清液和脱寡糖制备的生物侵蚀性增强了土着脱菱胚芽群，染色豌豆的旋转脱杆状血管

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摘要

Addition of living cells for plant growth promotion has several constraints including erratic and inconsistent field performance, poor shelf life, and reduced population due to tough competition with resident microbes. Due to the focus on employing cell-based formulation, bacterial cell free culture supernatant (CFCS), and metabolites produced by them, have been largely ignored. Hence, present study aimed to enhance productivity and nodulation of Cajanus cajan (pigeon pea) by using diverse formulations developed from Bradyrhizobium strain IC-4059 (cell based formulation), its CFCS (supernatant based), and exopolysaccharides (EPS) (metabolite based), blended formulation (cells with CFCS; cells with metabolite; metabolite with CFCS), and combinational formulation (IC-4059 + EPS + CFCS). Efficacy of developed formulations was monitored initially in tube assay, followed by field experiment with Pusa 991 pigeon pea cultivar in randomized block design. Growth promoting metabolites present in CFCS and EPS were analyzed by metabolomic approach using UPLC-MS and HPLC, respectively. In both tube assay and field experiment, seeds receiving treatment with combination of IC-4059 cells plus CFCS and EPS performed best, followed by blend IC-4059 CFCS + EPS, and IC-4059 cells + EPS, in terms of plant parameters. During field experiment seeds treated with combination showed significant enhancement in shoot length, root length, dry weight, protein content, and nodule number by 1.2, 1.3, 1.2, 1.1 and 2.0-fold, respectively, in comparison with rhizobia-based formulation. The prominent compounds identified by UPLC-MS were phosphatidylserine, phosphatidylcholine, FMC-5, pantoic acid, ascorbic acid, benzoic acid, 4-pentyl-4-formylphenyl ester, benzimidazole, and phosphatidylethanolamine. The study disseminates the use of combinational formulation developed from Bradyrhizobium sp. IC-4059, its CFCS, and its EPS for augmenting nodulation and growth of pigeon pea crop in field in comparison with application of rhizobial cell alone, as next generation of bioformulations.
机译：为植物生长促进的生物细胞增加了几个限制，包括不稳定和不一致的现场性能，差的保质期，并且由于与常规微生物的艰难的竞争而降低人口。由于专注于采用基于细胞的配方，因此在很大程度上忽略了细菌细胞无菌培养上清液（CFC）和由它们产生的代谢物。因此，目前的研究旨在通过使用从Bradyrhizobium菌株IC-4059（Cell基的制剂），其CFCs（基于上清液）和外奥多糖（EPS）开发的各种配方来提高Cajanus Cajan（Pigeon Pea）的生产率和培训。（基于代谢物） ，混合配方（具有CFCs的细胞;具有代谢物的细胞;与CFC的代谢物），以及组合制剂（IC-4059 + EPS + CFC）。最初监测发育配方的疗效，然后在管道测定中监测，然后进行帕斯卡991鸽子豌豆品种在随机砌块设计中的实验。通过使用UPLC-MS和HPLC分别通过代谢物方法分析促进CFC和EP中存在的代谢物的生长。在两管测定和田间实验中，使用IC-4059个细胞组合的种子接受治疗，并在植物参数方面表现最佳，其次是Blend IC-4059 CFCs + EPS和IC-4059细胞+ EPS。与基于根扎的制剂相比，在组合处理的野外实验中，分别在组合处理的种子中，分别显着增强了枝条长度，根长，干重，蛋白质含量和结节数。通过UPLC-MS鉴定的突出化合物是磷脂酰丝氨酸，磷脂酰胆碱，FMC-5，外皮酸，抗坏血酸，苯甲酸，4-戊基-4-甲酰基苯基酯，苯并咪唑和磷脂酰乙醇胺。该研究传播了从Bradyrhizobium SP开发的组合制剂的使用。与单独的Rhizobial细胞应用相比，IC-4059，其CFCs及其综合植物豆类作物的氧化术和生长的EPS，作为下一代生物运化。

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