Genetic stability of the attachment glycoprotein of human respiratory syncytial viruses during serial passages in cell cultures.

de Sierra M; Arbiza J

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Genetic stability of the attachment glycoprotein of human respiratory syncytial viruses during serial passages in cell cultures.

机译：人类呼吸道合胞病毒附着糖蛋白在细胞培养中连续传代过程中的遗传稳定性。

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Thirteen isolates of human respiratory syncytial viruses (HRSV) of groups A and B were isolated in HEp-2 cells from nasopharyngeal aspirates (NPA) from the children with acute respiratory infections. Three isolates of HRSV of group A were propagated in HEp-2 cells in 20 serial passages. Nucleotide sequences of the products obtained by RT-PCR from the glycoprotein (G) hypervariable region of the original virus isolates in NPA and those after one or several passages were compared. All the isolates analyzed showed no changes during passaging in HEp-2 cells.

机译：从患有急性呼吸道感染的儿童的鼻咽抽吸物（NPA）的HEp-2细胞中分离出13组A和B组人类呼吸道合胞病毒（HRSV）。 A组HRSV的三种分离株在HEp-2细胞中连续20代繁殖。比较了通过RT-PCR从NPA中原始病毒分离株的糖蛋白（G）高变区获得的产物的核苷酸序列，以及经过一或几次传代后的核苷酸序列。分析的所有分离株在HEp-2细胞传代过程中均未显示任何变化。

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《Acta Virologica: International Journal》 |2004年第2期|共7页
作者
de Sierra M; Arbiza J;
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正文语种 eng
中图分类基础医学;
关键词
Respiratory syncytial virus; Periodicals lt; 1gt; Human; Glycoproteins; 糖蛋白类;

机译：Respiratory syncytial virus;Periodicals 1;Human;Glycoproteins;糖蛋白类;

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1. Genetic stability of the attachment glycoprotein of human respiratory syncytial viruses during serial passages in cell cultures. [J] . de Sierra M, Arbiza J Acta Virologica: International Journal . 2004,第2期

机译：人类呼吸道合胞病毒附着糖蛋白在细胞培养中连续传代过程中的遗传稳定性。
2. Amino acid changes in the attachment G glycoprotein of human respiratory syncytial viruses (subgroup A) isolated in Italy over several epidemics (1997-2006). [J] . Montieri S, Puzelli S, Ciccozzi M, Journal of Medical Virology . 2007,第12期

机译：在意大利的几次流行病中（1997-2006年），人类呼吸道合胞病毒（A组）的附着G糖蛋白的氨基酸变化。
3. Genetic analysis of attachment glycoprotein (G) gene in new genotype ON1 of human respiratory syncytial virus detected in Japan [J] . Hiroyuki Tsukagoshi, Hajime Yokoi, Miho Kobayashi, Microbiology and Immunology . 2013,第9期

机译：日本检测到的人类呼吸道合胞病毒新基因ON1型糖蛋白（G）附着基因的遗传分析
4. Development and Pilot Study of a Dual-Target RSV Assay to Detect and Subtype Respiratory Syncytial Viruses in Nasal Swab Samples [C] . Marla Chu, Bita Parhy, Sam Park, International Molecular Medicine Tri-Conference. . 2016

机译：双靶RSV测定检测鼻拭子样本中的双靶RSV测定的开发和试验研究
5. Prevention of Respiratory Syncytial Virus Attachment Protein Cleavage in Vero Cells Rescues Infectivity of Progeny Virions for Primary Human Airway Cultures. [D] . Corry, Jacqueline D. 2015

机译：预防Vero细胞中呼吸道合胞病毒附着蛋白的切割可拯救子代病毒对原代人气道培养物的感染性。
6. Genetic Stability of Parainfluenza Virus 5-Vectored Human Respiratory Syncytial Virus Vaccine Candidates after In Vitro and In Vivo Passage [O] . Shannon I. Phan, Carolyn M. Adam, Zhenhai Chen, 2017

机译：体外和体内传代后副流感病毒5载体人类呼吸道合胞病毒疫苗候选者的遗传稳定性。
7. Genetic variation in attachment glycoprotein genes of human respiratory syncytial virus subgroups a and B in children in recent five consecutive years. [O] . Guanglin Cui, Runan Zhu, Yuan Qian, 2013

机译：近5年来儿童呼吸道合胞病毒亚型a和B附着糖蛋白基因的遗传变异。
8. Selection of Attenuated Dengue-4 Viruses by Serial Passage in Primary Kidney Cells. V. Human Response to Immunization with a Candidate Vaccine Prepared in Fetal Rhesus Lung Cells [R] . Eckels, K. H., Scott, R. M., Bancroft, W. H., 1984

机译：原发性肾细胞系列通道选择减毒登革4病毒。 V.用胎儿恒河猴肺细胞制备的候选疫苗对人体免疫反应

Genetic stability of the attachment glycoprotein of human respiratory syncytial viruses during serial passages in cell cultures.

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