• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Advanced drug delivery reviews >Two-photon excitation imaging of exocytosis and endocytosis and determination of their spatial organization.
【24h】

Two-photon excitation imaging of exocytosis and endocytosis and determination of their spatial organization.

机译：胞吐作用和胞吞作用的双光子激发成像及其空间组织的确定。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Two-photon excitation imaging is the least invasive optical approach to study living tissues. We have established two-photon extracellular polar-tracer (TEP) imaging with which it is possible to visualize and quantify all exocytic events in the plane of focus within secretory tissues. This technology also enables estimate of the precise diameters of vesicles independently of the spatial resolution of the optical microscope, and determination of the fusion pore dynamics at nanometer resolution using TEP-imaging based quantification (TEPIQ). TEP imaging has been applied to representative secretory glands, e.g., exocrine pancreas, endocrine pancreas, adrenal medulla and a pheochromocytoma cell line (PC12), and has revealed unexpected diversity in the spatial organization of exocytosis and endocytosis crucial for the physiology and pathology of secretory tissues and neurons. TEP imaging and TEPIQ analysis are powerful tools for elucidating the molecular and cellular mechanisms of exocytosis and certain related diseases, such as diabetes mellitus, and the development of new therapeutic agents and diagnostic tools.
机译：双光子激发成像是研究生物组织的侵入性最小的光学方法。我们已经建立了两光子细胞外极示踪剂（TEP）成像，通过它可以可视化和量化分泌组织内聚焦平面中的所有胞外事件。该技术还能够独立于光学显微镜的空间分辨率来估计囊泡的精确直径，并使用基于TEP成像的定量技术（TEPIQ）确定纳米分辨率下的融合孔动力学。 TEP成像已应用于代表性的分泌腺，例如外分泌胰腺，内分泌胰腺，肾上腺髓质和嗜铬细胞瘤细胞系（PC12），并且揭示了胞吐作用和内吞作用的空间组织中出乎意料的多样性，这对于分泌物的生理学和病理学至关重要组织和神经元。 TEP成像和TEPIQ分析是阐明胞吐作用和某些相关疾病（例如糖尿病）的分子和细胞机制以及开发新的治疗剂和诊断工具的强大工具。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号