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首页> 外文期刊>Archives of microbiology >Pseudomonas aestus sp nov., a plant growth-promoting bacterium isolated from mangrove sediments
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Pseudomonas aestus sp nov., a plant growth-promoting bacterium isolated from mangrove sediments

机译：Pseudomonasspsestus sp 11月。，植物生长促进从红树林沉积物中分离的细菌

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摘要

Strain CMAA 1215(T), a Gram-reaction-negative, aerobic, catalase positive, polarly flagellated, motile, rod-shaped (0.5-0.8 x 1.3-1.9 A mu m) bacterium, was isolated from mangrove sediments, Canan,ia Island, Brazil. Analysis of the 16S rRNA gene sequences showed that strain CMAA 1215(T) forms a distinct phyletic line within the Pseudomonas putida subclade, being closely related to P. plecoglossicida ATCC 700383(T), P. monteilii NBRC 103158(T), and P. taiwanensis BCRC 17751(T) of sequence similarity of 98.86, 98.73, and 98.71%, respectively. Genomic comparisons of the strain CMAA 1215(T) with its closest phylogenetic type strains using average nucleotide index (ANI) and DNA:DNA relatedness approaches revealed 84.3-85.3% and 56.0-63.0%, respectively. A multilocus sequence analysis (MLSA) performed concatenating 16S rRNA, gyrB and rpoB gene sequences from the novel species was related with Pseudomonas putida subcluster and formed a new phylogenetic lineage. The phenotypic, physiological, biochemical, and genetic characteristics support the assignment of CMAA 1215(T) to the genus Pseudomonas, representing a novel species. The name Pseudomonas aestus sp.nov. is proposed, with CMAA 1215(T) (=NRRL B-653100(T) = CBMAI 1962(T)) as the type strain.
机译：菌株CMAA 1215（T），克反应阴性，有氧，过氧化氢酶阳性，极性鞭打，动机，棒状（0.5-0.8×1.3-1.9 a mu m）细菌，从红树林沉积物中分离，米兰，IA岛屿，巴西。 16S RRNA基因序列的分析表明，菌株CMAA 1215（T）在普赖达亚核苷酸亚核苷酸内形成了不同的文学系，与P.Plecoglossicida ATCC 700383（T），P. Monteilii NBRC 103158（T）密切相关，和P 。序列相似性分别为98.86,98.73和98.71％的序列相似性。使用平均核苷酸指数（ANI）和DNA的菌株CMAA 1215（T）具有其最近的系统发育型菌株的基因组比较：DNA相关性方法分别显示出84.3-85.3％和56.0-63.0％。从新型物种中串联16S rRNA，GyrB和RPOB基因序列进行的多点序列分析（MLSA）与Pseudomonas pivera亚聚蛋白有关，形成了新的系统发育谱系。表型，生理学，生物化学和遗传特征支持CMAA 1215（T）的分配给伪霉素属，代表新颖的物种。名字假单胞菌艾斯特斯SP.Nov。提出，CMAA 1215（T）（= NRRL B-653100（T）= CBMAI 1962（T））作为类型应变。

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