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首页> 外文期刊>Archives of microbiology >A metabonomic analysis on the response of Enterobacter cloacae from coastal outfall for land-based pollutant under phoxim stress
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A metabonomic analysis on the response of Enterobacter cloacae from coastal outfall for land-based pollutant under phoxim stress

机译：山茱萸下陆基污染物沿海排出肠道裂口肠杆菌响应的代谢响应性分析

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摘要

Enterobacter cloacae is an opportunistic pathogen widely distributed in human and animal intestinal systems. The secretion of extended-spectrum beta-lactamases (ESBLs) and cephalosporinase (AmpC) endows E. cloacae with strong drug resistance. In a previous study by our group, protein expression of E. cloacae under phoxim stress was measured by two-dimensional electrophoresis. Here, nuclear magnetic resonance was used to detect differences in E. cloacae metabonomics when under phoxim stress. We determined that there are 29 types of metabolites that differ between phoxim stress and normal culture conditions. Among these, 6 types of metabolites were upregulated in the phoxim stress group, and 23 types of metabolites were inhibited. Though enrichment analysis, seven pathways were identified by different expression levels of metabolites, which were involved in DNA and RNA synthesis, DNA damage repair, antioxidation and functions of the cell membrane and cell wall. The mechanism underlying how phoxim affects E. cloacae was determined by studying the results of both two-dimensional electrophoresis in our prior work and the analysis of E. cloacae metabonomic changes under phoxim stress.
机译：肠杆菌是一种广泛分布在人和动物肠系中的机会主义病原体。扩展光谱β-内酰胺酶（ESBLS）和头孢菌素（AMPC）的分泌赋予E.Cloace具有强耐药性。在先前的研究中，通过二维电泳测量吡咯沉淀下的E.Cloacae的蛋白质表达。这里，核磁共振用于在磷脂骨胁迫下检测E.Cloacae代谢物理学的差异。我们确定有29种类型的代谢产物，含有磷脂含量和正常培养条件之间的代谢物。其中，在Phoxim应激基团中上调了6种类型的代谢物，抑制了23种代谢物。虽然富集分析，通过不同的表达水平鉴定了七种途径，其代谢产物涉及DNA和RNA合成，DNA损伤修复，抗氧化和细胞壁的抗氧化和功能。通过研究我们在我们的前后工作中的二维电泳的结果和磷脂酰基代谢氏族变化的分析来确定Phoxim如何影响E.Cloacae的机制。

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  • 来源
    《Archives of microbiology》 |2017年第8期|共9页
  • 作者

    Zhang Dijun; He Shan; Ming Tinghong; Lu Chenyang; Zhou Jun; Su Xiurong;

  • 作者单位

    Ningbo Univ Sch Marine Sci 818 Fenghua Rd Ningbo 315211 Zhejiang Peoples R China;

    Ningbo Univ Sch Marine Sci 818 Fenghua Rd Ningbo 315211 Zhejiang Peoples R China;

    Ningbo Univ Sch Marine Sci 818 Fenghua Rd Ningbo 315211 Zhejiang Peoples R China;

    Ningbo Univ Sch Marine Sci 818 Fenghua Rd Ningbo 315211 Zhejiang Peoples R China;

    Ningbo Univ Sch Marine Sci 818 Fenghua Rd Ningbo 315211 Zhejiang Peoples R China;

    Ningbo Univ Sch Marine Sci 818 Fenghua Rd Ningbo 315211 Zhejiang Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 微生物学;
  • 关键词

    Enterobacter cloacae; Phoxim; H-1 NMR; Metabonomic;

    机译：肠杆菌肝脏;Phoxim;H-1 NMR;代谢组合;

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