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首页> 外文期刊>Archives of microbiology >Identification of proteins differentially expressed by Chlamydia trachomatis treated with chlamydiaphage capsid protein VP1 during intracellular growth
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Identification of proteins differentially expressed by Chlamydia trachomatis treated with chlamydiaphage capsid protein VP1 during intracellular growth

机译：在细胞内生长期间用衣原体胶囊蛋白VP1治疗型衣原体囊瘤癌的蛋白质鉴定

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摘要

Chlamydia trachomatis infection is one of the most prevalent sexually transmitted diseases. Our research pertains to the inhibitory effect and molecular mechanism of the chlamydiaphage capsid protein VP1 on the growth of Chlamydia trachomatis. In this research, the capsid protein VP1 of the guinea-pig conjunctivitis chlamydiaphage phiCPG1 was expressed, purified and identified, and then, it was applied to the cultivation of different serovars of Chlamydia trachomatis and Chlamydia psittaci. The inhibitory effect was observed in each serovar of Chlamydia trachomatis (D, E, F, G, H, I, K, and L2) and Chlamydia psittaci inoculated with VP1 protein. The inhibition affection of VP1 on the growth of Chlamydia trachomatis was caused by the changes of expressions of some related proteins including 36 proteins up-regulated and 81 proteins down-regulated in the development cycle of Ct through the label-free test, and the transcription levels of these proteins, including Hc1, pmpD, and MOMP, were confirmed by RT-PCR. It provides information that is essential for understanding the mechanism of chlamydiaphage capsid protein VP1 on chlamydia and a new direction for further clinical treatment of chlamydial infection.
机译：Chlamydia Thachomatis感染是最普遍的性传播疾病之一。我们的研究涉及衣原体辣椒囊蛋白VP1对衣原体衣原体衣原体的抑制作用和分子机制。在这项研究中，表达，纯化和鉴定了几内亚猪结膜炎衣原体pHICPG1的辣椒蛋白VP1，然后纯化和鉴定，纯化和鉴定出来的衣原体衣原体和衣原体Psittaci的不同血清素。在每个衣原体衣原体（D，E，F，G，H，I，K和L2）中观察到抑制作用（D，E，F，G，H，I，K和L2）和接种与VP1蛋白的衣原体psittaci。 VP1对衣原体粉碎瘤的生长的抑制作用是由一些相关蛋白质的表达的变化引起的，其中包括36种蛋白质上调和81个蛋白质在CT的发育循环中通过无标记测试和转录来调节。通过RT-PCR确认包括HC1，PMPD和MOMP的这些蛋白质的水平。它提供了了解理解衣原体衣原体蛋白VP1对衣原体的机制至关重要的信息，以及用于进一步临床治疗衣原体感染的新方向。

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  • 来源
    《Archives of microbiology》 |2017年第8期|共11页
  • 作者

    Ma Jingyue; Sun Yina; Sun Changgui; Zhou Quan; Qi Manli; Kong Jie; Wang Jing; Liu Yuanjun; Liu Quanzhong;

  • 作者单位

    Tianjin Med Univ Dept Dermatovenereol Gen Hosp 154 Anshan Rd Tianjin 300052 Peoples R China;

    Tianjin Med Univ Metab Dis Hosp Key Lab Hormones &

    Dev Minist Hlth 22 Qixiangtai Rd Tianjin;

    Tianjin Med Univ Dept Dermatovenereol Gen Hosp 154 Anshan Rd Tianjin 300052 Peoples R China;

    Tianjin Med Univ Dept Dermatovenereol Gen Hosp 154 Anshan Rd Tianjin 300052 Peoples R China;

    Tianjin Med Univ Dept Dermatovenereol Gen Hosp 154 Anshan Rd Tianjin 300052 Peoples R China;

    Tianjin Med Univ Dept Dermatovenereol Gen Hosp 154 Anshan Rd Tianjin 300052 Peoples R China;

    Tianjin Med Univ Dept Dermatovenereol Gen Hosp 154 Anshan Rd Tianjin 300052 Peoples R China;

    Tianjin Med Univ Dept Dermatovenereol Gen Hosp 154 Anshan Rd Tianjin 300052 Peoples R China;

    Tianjin Med Univ Dept Dermatovenereol Gen Hosp 154 Anshan Rd Tianjin 300052 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 微生物学;
  • 关键词

    Chlamydia trachomatis; Chlamydiaphages; VP1; Inhibition;

    机译：Chlamydia Thachomatis;衣原体;VP1;抑制;

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