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Analysis of a non-canonical NtcA-dependent promoter in Synechococcus elongatus and its regulation by NtcA and P-II

机译:NTCA和P-II中综合球菌的非规范NTCA依赖性启动子分析及其调节

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摘要

In this communication, we present a genetic analysis of the glnN promoter region of Synechococcus elongatus PCC 7942. luxAB reporter fusions were used to characterize the glnN promoter by deletion and site-directed mutational analysis. Reporter gene expression analysis was performed in S. elongatus wild-type and mutant strains to reveal the role of the global nitrogen responsive transcription factor NtcA and of the P-II signalling protein on regulation of glnN gene expression. A non-canonical NtcA-binding motif is responsible for NtcA-dependent, nitrogen-responsive regulation of glnN. The P-II signalling protein has opposing effects on NtcA-dependent glnN expression. Under conditions of nitrate-growth, it depresses expression, whereas under conditions of combined nitrogen starvation, it is required for full induction. Furthermore, sequences upstream of the NtcA-binding site have repressive effect on glnN promoter activity.
机译:在这种通信中,我们介绍了SyneChocccus Elongatus PCC 7942的Glnn启动子区的遗传分析。Luxab报告者融合用于通过缺失和定向的突变分析表征GlnN启动子。 记者基因表达分析在S.Elongatus野生型和突变菌株中进行,揭示全局氮响应转录因子NTCA和P-II信号蛋白的作用对GLNN基因表达的调节。 非规范的NTCA结合基质是对GLNN的NTCA依赖性的氮响应调节负责。 P-II信号蛋白对NTCA依赖性GLNN表达具有相反的作用。 在硝酸盐生长的条件下,抑制表达,而在合并氮饥饿的条件下,需要完全诱导。 此外,NTCA结合位点上游的序列对GLNN启动子活性具有抑制作用。

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